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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/42163
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dc.creatorLuciana Fachini Costapt_BR
dc.creatorMarcos Xavier Silvapt_BR
dc.creatorTatiane Alves Paixãopt_BR
dc.creatorRenato Lima Santospt_BR
dc.creatorAnna Christina de Almeidapt_BR
dc.creatorMoises Sena Pessoapt_BR
dc.creatorLais Bitencourt Guimarãespt_BR
dc.creatorAnne Karoene Silva Fariapt_BR
dc.creatorRodrigo Pereira Morãopt_BR
dc.creatorJuliana Pinto da Silva Molpt_BR
dc.creatorLuize Néli Nunes Garciapt_BR
dc.creatorAnna Christina de Almeidapt_BR
dc.creatorAurora Maria Guimarães Gouveiapt_BR
dc.date.accessioned2022-06-01T14:32:58Z-
dc.date.available2022-06-01T14:32:58Z-
dc.date.issued2016-
dc.citation.volume9pt_BR
dc.citation.issue190pt_BR
dc.identifier.doiDOI 10.1186/s13104-016-1998-2pt_BR
dc.identifier.issn1756-0500pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/42163-
dc.description.resumoBackground: Brucella ovis infection is one of the leading causes of sub fertility and infertility in ovine, been characterized mainly by epididymitis, orchitis and testicular atrophy in rams. This study aimed to determine the frequency of B. ovis positivity in rams and goats flocks in the State of Minas Gerais, Brazil, by agarose gel immunodiffusion (AGID), ELISA, Rose Bengal, PCR and bacteriological isolation as diagnostic tools. Findings: Serum and urine samples were collected from properties with sheep or goat flocks, or from properties with mixed flock. Out of 50 sheep flocks, 6 % (3/50) were seropositive by AGID while 4 % (2/50) were positive by urine PCR for B. ovis. Out of five goat farms, 20 % (1/5) were seropositive for B. ovis by AGID. Mixed flock farms had 11.1 % (2/18) of positivity by AGID. By ELISA, 19.5 % (8/41) of sheep properties and 61.1 % (11/18) of the properties with mixed flocks were positive for B. ovis. No samples were positive in the test of Rose Bengal, ruling out exposure to smooth LPS Brucella species (particularly Brucella melitensis) and indicating that the positive in the ELISA was associated with Brucella spp. LPS rough (presumably B. ovis). No urine sample from sheep or goat was positive by bacteriological isolation. Conclusions: Our results demonstrate serologic or molecular evidence of B. ovis infection in several rams and billy goats from meso-regions of the State of Minas Gerais, Brazil. Also, this study report the indirect ELISA as an important tool for the diagnosis of B. ovis infection, as indirect ELISA in this study demonstrated to be the most sensitive diagnostic method adopted.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIASpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofBMC Research Notespt_BR
dc.rightsAcesso Abertopt_BR
dc.subject.otherColibacilosept_BR
dc.subject.otherBrucelosept_BR
dc.subject.otherOvinopt_BR
dc.subject.otherCaprinopt_BR
dc.titleSerologic and molecular evidence of Brucella ovis infection in ovine and caprine flocks in the State of Minas Gerais, Brazilpt_BR
dc.typeArtigo de Periódicopt_BR
dc.identifier.orcidhttps://orcid.org/ 0000-0001-9836-4117pt_BR
Appears in Collections:Artigo de Periódico

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