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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/45442
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dc.creatorCaroline Christine Santa Rosapt_BR
dc.creatorMarcela Marçal Thebitpt_BR
dc.creatorKamilla Faria Macielpt_BR
dc.creatorLuciana Carla Neves Britopt_BR
dc.creatorLeda Quércia Vieirapt_BR
dc.creatorAntônio Paulino Ribeiro Sobrinhopt_BR
dc.date.accessioned2022-09-24T16:55:30Z-
dc.date.available2022-09-24T16:55:30Z-
dc.date.issued2018-11-29-
dc.citation.volume32pt_BR
dc.citation.spage1pt_BR
dc.citation.epage7pt_BR
dc.identifier.doi10.1590/1807-3107bor-2018.vol32.0120pt_BR
dc.identifier.issn18073107pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/45442-
dc.description.resumoThe present study aims to evaluate the longitudinal effects of induced experimental infections in gnotoxenic animals on the expression of inflammatory chemokines and their receptors in periradicular tissues. The null hypothesis tested was that Enterococcus faecalis and Fusobacterium nucleatum had no effect on CCR5, CCL5, CXCL10, CCL2/MCP-1, CXCR2 and CCR1 expression. Two groups of five animals (n = 5) aged between 8 and 12 weeks were used in this study. The animals were anaesthetized, and coronary access was performed in the first molar on the right and left sides. Microorganisms were inoculated into the left molar, and the right molar was sealed without contamination to function as a control. Animals were sacrificed 7 and 14 days after infection, and periapical tissues were collected. The cytokine mRNA expression levels were assessed using realtime PCR. The chemokine mRNA expression levels demonstrated that the experimental infection was capable of inducing increased chemokine expression on day 7 compared to that on day 14, except for CCR5 and CCL5, which showed no changes. The gnotoxenic animal model proved to be effective and allowed evaluation of the immune response against a known infection. Additionally, this study demonstrates that gene expression of chemokines and their receptors against the experimental infection preferentially prevailed during the initial phase of induction of the periradicular alteration (i.e., on day 7 post-infection)pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofBrazilian Oral Researchpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectGerm-free lifept_BR
dc.subjectChemonikespt_BR
dc.subjectPeriapical Diseasespt_BR
dc.subjectMicrobiologypt_BR
dc.subject.otherGerm-free lifept_BR
dc.subject.otherPeriapical Diseasespt_BR
dc.subject.otherChemokinespt_BR
dc.subject.otherInfectionspt_BR
dc.titleEvaluation of chemokines and receptors in gnotobiotic root canal infection by f. nucleatum and e. faecalispt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.scielo.br/j/bor/a/63hGYMdnhYmRmSbfyv4ZswB/?lang=enpt_BR
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