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http://hdl.handle.net/1843/45492| Type: | Artigo de Periódico |
| Title: | In vivo analysis of the presence of heme oxygenase-1, transcription factor Jun-D and CD90+/CD73+/CD105+/CD45- cells in the pulp of bleached teeth |
| Authors: | Francine Benetti André Luiz Fraga Briso Araújo Lopes Carminatti Conti Marjorie de Oliveira Gallinari Edilson Ervolino Luciano Tavares Angelo Cintra |
| Abstract: | AimTo investigate hydrogen peroxide (H2O2)-inducedresponsiveness in pulp cells using heme oxygenase-1(HO-1) immunolabelling, Jun-D immunolabelling tostudy the effects of H2O2on odontoblastic differentiationand CD90+/CD73+/CD105+/CD45 cell counting forin vivoidentification of mesenchymal stem cells in thepulp.MethodologyThe maxillary molars of 50 rats weretreated with a bleaching gel (35% H2O2,1930 min) orplacebo gel (control groups). At 2, 3, 7, 15 and 30 daysafter the treatment (n=10), inflammation in pulp tissuewas analysed by haematoxylin–eosin staining, HO-1-and Jun-D-immunolabelled cells were counted ineach third of the pulp chamber, and the number ofCD90+/CD73+/CD105+/CD45 cells was quantified byimmunofluorescence. The results were assessed using thePairedt-test or Wilcoxon signed-rank test (P<0.05).ResultsSignificant H2O2-induced inflammation wasnoted at 2 and 3 days (P<0.05), with tertiary den-tine formation occurring from 7 days. The bleachedspecimens had greater HO-1 immunolabelling in themiddle and cervical thirds of the coronal pulp at 2and 3 days, in all thirds at 7 days, and in the occlu-sal third at 15 days (P<0.05), and significantnuclear Jun-D immunolabelling in the cervical thirdat 2 and 3 days and in the occlusal and middle thirdsat 7 days (P<0.05). Bleached and control groupshad low numbers of CD90+/CD73+/CD105+/CD45 cells in the pulp at all periods (P>0.05).ConclusionsPulp cells responded to oxidative stressby expressing HO-1 during the post-bleaching inflam-mation phase until the beginning of the repair phase.Jun-D expression occurred during the reduction ofinflammation and the beginning of tertiary dentine production. The presence of oxidative stress did notinfluence the number of CD90+/CD73+/CD105+/CD45 cells identifiedin vivo in the dental pulp |
| Subject: | Dental pulp cavity Tooth bleaching Heme oxygenase-1 Hydrogen peroxide Stem cells |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | https://doi.org/10.1111/iej.13190 |
| URI: | http://hdl.handle.net/1843/45492 |
| Issue Date: | 19-Jul-2019 |
| metadata.dc.url.externa: | https://onlinelibrary.wiley.com/doi/full/10.1111/iej.13190 |
| metadata.dc.relation.ispartof: | International Endodontic Journal |
| Appears in Collections: | Artigo de Periódico |
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