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Type:	Artigo de Periódico
Title:	Trypanosoma cruzi needs a signal provided by reactive oxygen species to infect macrophages
Other Titles:	Trypanosoma cruzi precisa de um sinal fornecido por espécies reativas de oxigênio para infectar macrófagos
Authors:	Grazielle Ribeiro Goes Peter Silva Rocha Aline r. s. Diniz Pedro Henrique Nascimento de Aguiar Carlos Renato Machado Leda Quercia Vieira
Abstract:	Background: During Trypanosoma cruzi infection, macrophages produce reactive oxygen species (ROS) in a process called respiratory burst. Several works have aimed to elucidate the role of ROS during T. cruzi infection and the results obtained are sometimes contradictory. T. cruzi has a highly efficiently regulated antioxidant machinery to deal with the oxidative burst, but the parasite macromolecules, particularly DNA, may still suffer oxidative damage. Guanine (G) is the most vulnerable base and its oxidation results in formation of 8-oxoG, a cellular marker of oxidative stress. Methodology/Principal Findings: In order to investigate the contribution of ROS in T. cruzi survival and infection, we utilized mice deficient in the gp91phox (Phox KO) subunit of NADPH oxidase and parasites that overexpress the enzyme EcMutT (from Escherichia coli) or TcMTH (from T. cruzi), which is responsible for removing 8-oxo-dGTP from the nucleotide pool. The modified parasites presented enhanced replication inside murine inflammatory macrophages from C57BL/6 WT mice when compared with control parasites. Interestingly, when Phox KO macrophages were infected with these parasites, we observed a decreased number of all parasites when compared with macrophages from C57BL/6 WT. Scavengers for ROS also decreased parasite growth in WT macrophages. In addition, treatment of macrophages or parasites with hydrogen peroxide increased parasite replication in Phox KO mice and in vivo. Conclusions: Our results indicate a paradoxical role for ROS since modified parasites multiply better inside macrophages, but proliferation is significantly reduced when ROS is removed from the host cell. Our findings suggest that ROS can work like a signaling molecule, contributing to T. cruzi growth inside the cells.
Subject:	Trypanosoma cruzi Doença de chagas Espécies reativas de nitrogênio Espécies reativas de oxigênio
language:	eng
metadata.dc.publisher.country:	Brasil
Publisher:	Universidade Federal de Minas Gerais
Publisher Initials:	UFMG
metadata.dc.publisher.department:	ICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
Rights:	Acesso Aberto
metadata.dc.identifier.doi:	https://doi.org/10.1371/journal.pntd.0004555
URI:	http://hdl.handle.net/1843/55303
Issue Date:	2016
metadata.dc.url.externa:	https://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004555
metadata.dc.relation.ispartof:	PLoS Neglected Tropical Diseases
Appears in Collections:	Artigo de Periódico

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