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http://hdl.handle.net/1843/60177| Type: | Artigo de Periódico |
| Title: | Optimal cytokinin/auxin balance for indirect shoot organogenesis of Eucalyptus cloeziana and production of ex vitro rooted micro-cuttings |
| Authors: | Leandro Silva de Oliveira Gilvano Ebling Brondani Letícia Vaz Molinari Rafaella Zanetti Dias Gustavo Leal Teixeira Antônio Natal Gonçalves Marcílio de Almeida |
| Abstract: | Shoot organogenesis is critical for the shortening of long breeding cycles and circumvent the barrier of cloning mature Eucalyptus cloeziana trees. It enables large-scale production of plants from transformed tissues. This study evaluates the effect of α-naphthaleneacetic acid (NAA), thidiazuron (TDZ) and benzylaminopurine (BAP) on the organogenesis of E. cloeziana from hypocotyls and cotyledonary leaves. In the induction stage, hypocotyls and cotyledonary leaves were established in a Murashige and Skoog (MS) culture medium supplemented with NAA or TDZ. Callus tissues were cultivated in a MS culture medium containing only BAP or different concentrations of BAP/NAA in the differentiation stage. Adventitious buds were multiplied in vitro and elongated in a WPM culture medium supplemented with 0.89 μM BAP and 0.05 μM NAA. Cot- yledonary leaves exhibited the best in vitro regeneration. The induction of adventitious buds occurred only in calluses induced from TDZ. In the differentiation stage, 4.4 μM BAP treatment promoted an increase of adventitious bud regeneration. Micro-cuttings from regenerated shoots were acclimatized and rooted ex vitro in mini-incubators. The results confirm the establishment of an efficient protocol for the in vitro regeneration of E. cloeziana by indirect organogenesis, providing new insights regarding cloning of this species. |
| Subject: | Plantas - Propagação in vitro Plantas - Reguladores Eucalipto |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS |
| Rights: | Acesso Restrito |
| URI: | http://hdl.handle.net/1843/60177 |
| Issue Date: | 2022 |
| metadata.dc.url.externa: | https://doi.org/10.1007/s11676-022-01454-9 |
| metadata.dc.relation.ispartof: | Journal of Forestry Research |
| Appears in Collections: | Artigo de Periódico |
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