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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/60177
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dc.creatorLeandro Silva de Oliveirapt_BR
dc.creatorGilvano Ebling Brondanipt_BR
dc.creatorLetícia Vaz Molinaript_BR
dc.creatorRafaella Zanetti Diaspt_BR
dc.creatorGustavo Leal Teixeirapt_BR
dc.creatorAntônio Natal Gonçalvespt_BR
dc.creatorMarcílio de Almeidapt_BR
dc.date.accessioned2023-10-27T18:49:38Z-
dc.date.available2023-10-27T18:49:38Z-
dc.date.issued2022-
dc.citation.volume33pt_BR
dc.citation.spage1573pt_BR
dc.citation.epage1584pt_BR
dc.identifier.issn1007-662Xpt_BR
dc.identifier.urihttp://hdl.handle.net/1843/60177-
dc.description.resumoShoot organogenesis is critical for the shortening of long breeding cycles and circumvent the barrier of cloning mature Eucalyptus cloeziana trees. It enables large-scale production of plants from transformed tissues. This study evaluates the effect of α-naphthaleneacetic acid (NAA), thidiazuron (TDZ) and benzylaminopurine (BAP) on the organogenesis of E. cloeziana from hypocotyls and cotyledonary leaves. In the induction stage, hypocotyls and cotyledonary leaves were established in a Murashige and Skoog (MS) culture medium supplemented with NAA or TDZ. Callus tissues were cultivated in a MS culture medium containing only BAP or different concentrations of BAP/NAA in the differentiation stage. Adventitious buds were multiplied in vitro and elongated in a WPM culture medium supplemented with 0.89 μM BAP and 0.05 μM NAA. Cot- yledonary leaves exhibited the best in vitro regeneration. The induction of adventitious buds occurred only in calluses induced from TDZ. In the differentiation stage, 4.4 μM BAP treatment promoted an increase of adventitious bud regeneration. Micro-cuttings from regenerated shoots were acclimatized and rooted ex vitro in mini-incubators. The results confirm the establishment of an efficient protocol for the in vitro regeneration of E. cloeziana by indirect organogenesis, providing new insights regarding cloning of this species.pt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIASpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of Forestry Research-
dc.rightsAcesso Restritopt_BR
dc.subjectMicropropagationpt_BR
dc.subjectRecalcitrant speciespt_BR
dc.subjectPlant growth regulatorpt_BR
dc.subjectExplants sourcept_BR
dc.subject.otherPlantas - Propagação in vitropt_BR
dc.subject.otherPlantas - Reguladorespt_BR
dc.subject.otherEucaliptopt_BR
dc.titleOptimal cytokinin/auxin balance for indirect shoot organogenesis of Eucalyptus cloeziana and production of ex vitro rooted micro-cuttingspt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://doi.org/10.1007/s11676-022-01454-9pt_BR
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