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http://hdl.handle.net/1843/60209| Tipo: | Artigo de Periódico |
| Título: | Integrated proteomics, phosphoproteomics and metabolomics analyses reveal similarities among giant cell granulomas of the jaws with different genetic mutations |
| Autor(es): | Jéssica Gardone Vitório Liséte Celina Lange Lucilaine Valéria de Souza Santos Martin Røssel Larsen Carolina Cavalieri Gomes Ricardo Santiago Gomez Filipe Fideles Duarte-Andrade Thais dos Santos Fontes Pereira Marcella Nunes de Melo Braga Gisele André Baptista Canuto Adriana Nori de Macedo Yuri Abner Rocha Lebron Victor Rezende Moreira Liza Figueiredo Felicori |
| Resumo: | Background: Giant cell granuloma of the jaws are benign osteolytic lesions of the jaws. These lesions are genetically characterized by mutually exclusive somatic mutations at TRPV4, KRAS, and FGFR1, and a fourth molecular subgroup which is wild-type for the three mutations. Irrespective of the molecular background, giant cell granulomas show MAPK/ERK activation. However, it remains unclear if these mutations lead to differences in their molecular signaling in giant cell granulomas. Methods: Metabolomics, proteomics, and phosphoproteomics analyses were carried out in formalin-fixed paraffin-embedded samples of giant cell granuloma of the jaws. The study cohort consisted of five lesions harboring mutations in FGFR1, six in KRAS, five in TRPV4, and five that were wild-type for these mutations. Results: Lesions harboring KRAS or FGFR1 mutations showed overall similar proteomics and metabolomics profiles. In all four groups, metabolic pathways showed similarity in apoptosis, cell signaling, gene expression, cell differentiation, and erythrocyte activity. Lesions harboring TRPV4 mutations showed a greater number of enriched pathways related to tissue architecture. On the other hand, the wild-type group presented increased number of enriched pathways related to protein metabolism compared to the other groups. Conclusion: Despite some minor differences, our results revealed an overall similar molecular profile among the groups with different mutational profile at the metabolic, proteic, and phosphopeptidic levels. |
| Assunto: | Biologia molecular Células - Patogênese Tumores Preparação de amostra (Química) Granuloma |
| Idioma: | eng |
| País: | Brasil |
| Editor: | Universidade Federal de Minas Gerais |
| Sigla da Instituição: | UFMG |
| Departamento: | ICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA ICB - DEPARTAMENTO DE MORFOLOGIA ICB - DEPARTAMENTO DE PATOLOGIA ICX - DEPARTAMENTO DE QUÍMICA |
| Tipo de Acesso: | Acesso Restrito |
| Identificador DOI: | https://doi.org/10.1111/jop.13327 |
| URI: | http://hdl.handle.net/1843/60209 |
| Data do documento: | 2022 |
| metadata.dc.url.externa: | https://onlinelibrary.wiley.com/doi/10.1111/jop.13327 |
| metadata.dc.relation.ispartof: | Journal of Oral Pathology & Medicine |
| Aparece nas coleções: | Artigo de Periódico |
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