Use este identificador para citar ou linkar para este item: http://hdl.handle.net/1843/63427
Tipo: Artigo de Periódico
Título: Testosterone improves the osteogenic potential of a composite in vitro and in vivo
Autor(es): Kelen Jorge Rodrigues da Costa Nunes
Alfonso Gala García
Joel Jose Passos
Vagner Rodrigues Santos
Rubén Dario Sinisterra Millán
Célia Regina Moreira Lanza
María Esperanza Cortés Segura
Resumo: Testosterone (T) has been suggested as a promising agent in the bone osteointegration when incorporated in a bioceramic/polymer combination for the local application. The objective of this study was to evaluate the activity of a testosterone composite of poly (lactic-co-glycolic acid) (PLGA), polycaprolactone (PCL), and biphasic calcium phosphate (BCP) as a strategy for enhancing its osteogenic effect and to evaluate tissue response to the composite implantation. PLGA/PCL/BCP/T and PLGA/PCL/BCP composites were prepared and characterized using thermal analysis. Composite morphology and surface characteristics were assessed by SEM and EDS. The evaluations of in vitro effects of testosterone composite on osteoblasts viability, alkaline phosphatase activity, collagen production, osteocalcin concentration, quantification of mineralization, and nitric oxide concentration, after 7, 14, and 21 days. Testosterone was successfully incorporated and composites showed a homogeneously distributed porous structure. The PLGA/PCL/BCP/T composite had a stimulatory effect on osteoblastic activity on the parameters evaluated, except to nitric oxide production. After 60 days, the PLGA/PCL/BCP/T composite showed no chronic inflammatory infiltrate, whereas the PLGA/PCL/BCP composite showed mild chronic inflammatory infiltrate. Angiogenesis, cellular adsorption, and fibrous deposit were observed on the surfaces of implanted composites. The composites in combination with testosterone can be exploited to investigate the use of this scaffold for bone integration.
Assunto: Testosterona
Fosfato de cálcio
Biomineralização
Ossos - Crescimento
Osseointegração
Idioma: eng
País: Brasil
Editor: Universidade Federal de Minas Gerais
Sigla da Instituição: UFMG
Departamento: FAO - DEPARTAMENTO DE CLÍNICA
FAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
ICX - DEPARTAMENTO DE QUÍMICA
Tipo de Acesso: Acesso Restrito
Identificador DOI: https://doi.org/10.1007/s00441-018-2970-3
URI: http://hdl.handle.net/1843/63427
Data do documento: 2019
metadata.dc.url.externa: https://link.springer.com/article/10.1007/s00441-018-2970-3
metadata.dc.relation.ispartof: Cell and Tissue Research
Aparece nas coleções:Artigo de Periódico

Arquivos associados a este item:
Não existem arquivos associados a este item.


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.