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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/64796
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dc.creatorTales Sambrano Vieirapt_BR
dc.creatorAna Paula Pintopt_BR
dc.creatorGabriela m Batituccipt_BR
dc.creatorAlisson L. Rochapt_BR
dc.creatorHugo T. Filhopt_BR
dc.creatorDawit Albieiro Pinheiro Gonçalvespt_BR
dc.creatorAdelino S. Silvapt_BR
dc.creatorEllen Cristini de Freitaspt_BR
dc.date.accessioned2024-02-27T16:19:47Z-
dc.date.available2024-02-27T16:19:47Z-
dc.date.issued2020-
dc.citation.volume63pt_BR
dc.citation.issue4pt_BR
dc.citation.spage171pt_BR
dc.citation.epage178pt_BR
dc.identifier.doihttps://doi.org/10.4103/CJP.CJP_31_20pt_BR
dc.identifier.issn0304-4920pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/64796-
dc.description.resumoDuring overnight sleep, the longest postabsorptive and inactive phase of the day causes protein catabolism and loss. However, the daytime ingestion of dairy proteins has been shown to stimulate muscle protein synthesis and growth. This study compared the effects of pre-sleep supplementation of a protein blend (PB) composed of micellar casein (MCa) and whey protein (1:1) versus isolate MCa on the plasma levels of branched-chain amino acids (BCAAs) and the activation of the mechanistic target of rapamycin (mTOR) signaling, a critical intracellular pathway involved in the regulation of muscle protein synthesis. After 10 h of fasting during the active phase, rats were fed with a single dose of PB or MCa (5.6 g protein/kg of body mass) by gavage, and samples of blood and gastrocnemius muscle were collected at 30, 90, and 450 min. PB and MCa supplementations induced an increase (~3-fold, P < 0.001) of plasma BCAAs at 30 and 90 min. Most importantly, the stimulatory phosphorylation levels of mTOR and its downstream target p70 ribosomal protein S6 kinase (p70S6K) were similarly higher (~2.5-fold, P < 0.001) 30 and 90 min after MCa and PB. Plasma levels of leucine, isoleucine, valine, and overall BCAAs were correlated with the activation of mTOR (P < 0.001) and p70S6K (P < 0.001). MCa and PB supplementations before the inactive phase of rats resulted in an anabolic milieu in the skeletal muscle by inducing a transient increase in plasma BCAAs and a similar activation of the mTOR/p70S6K axis.pt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorshipFAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentEEF - DEPARTAMENTO DE EDUCAÇÃO FÍSICApt_BR
dc.publisher.departmentEEFFTO - ESCOLA DE EDUCAÇÃO FISICA, FISIOTERAPIA E TERAPIA OCUPACIONALpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of Physiological Investigationpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectMicellar caseinpt_BR
dc.subjectMTOR signaling pathwaypt_BR
dc.subjectProtein supplementationpt_BR
dc.subjectProtein synthesispt_BR
dc.subjectSkeletal musclept_BR
dc.subjectWhey proteinpt_BR
dc.subject.otherProteínaspt_BR
dc.subject.otherProteínas - Pesquisapt_BR
dc.subject.otherProteínas - Síntesept_BR
dc.titleProtein Blend and Casein Supplementations before Inactive Phase Similarly Activate Mechanistic Target of Rapamycin Signaling in Rat Skeletal Musclept_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://journals.lww.com/cjop/fulltext/2020/63040/protein_blend_and_casein_supplementations_before.4.aspxpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-2621-3330pt_BR
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