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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/76838
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dc.creatorDenise Rubinho Dos Santos Martinspt_BR
dc.creatorCaroline Honaiser Lescanopt_BR
dc.creatorAlberto Fernando Oliveira Justopt_BR
dc.creatorJulia Modesto Vicentept_BR
dc.creatorSérgio Henrique Sousa Santospt_BR
dc.creatorCharles Martins Aguilarpt_BR
dc.creatorAlexandre Borgespt_BR
dc.creatorIvan Pires de Oliveirapt_BR
dc.creatorEliana Janet Sanjinez-Argandoñapt_BR
dc.date.accessioned2024-09-24T12:10:12Z-
dc.date.available2024-09-24T12:10:12Z-
dc.date.issued2023-
dc.citation.volume78pt_BR
dc.citation.issue2pt_BR
dc.citation.spage342pt_BR
dc.citation.epage350pt_BR
dc.identifier.doihttps://doi.org/10.1007/s11130-023-01067-5pt_BR
dc.identifier.issn09219668pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/76838-
dc.description.resumoHibiscus sabdariffa L. is a worldwide component for tea and beverages, being a natural source of anthocyanins, which are associated with cardiovascular activities. To investigate this relationship, we explored different methods of aqueous extraction on the anthocyanin content and antioxidant activity of H. sabdariffa L. calyx extract (HSCE). Pharmacological effects via platelet aggregation, calcium mobilization, cyclic nucleotide levels, vasodilator-stimulated phosphoprotein Ser157 and Ser239, and on the vasomotor response of aortic rings isolated from mice are studied herewith. We found that the application of ultrasonic turbolization, 20 min, combined with acidified water was significantly more effective in the extraction process, providing extracts with the highest levels of anthocyanins (8.73 and 9.63 mg/100 g) and higher antioxidant activity (6.66 and 6.78 μM trolox/g of sample). HSCE significantly inhibited (100-1000 μg/mL) arachidonic acid-induced platelet aggregation, reduced calcium mobilization, and increased cAMP and cGMP levels with VASPSer157 and VASPSer239 phosphorylation. Vasorelaxation reduction was confirmed by the aortic rings and endothelium assays treated with nitric oxide synthase inhibitors, soluble guanylyl cyclase (sGC) oxidizing agent, or Ca2+-activated K+ channel inhibitor. The increasing of cGMP levels could be understood considering the sGC stimulation by HSCE compounds in the specific stimulus domain, which allows an understanding of the observed antiplatelet and vasorelaxant properties of H. sabdariffa L. calyx extract.pt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipFAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulopt_BR
dc.description.sponsorshipOutra Agênciapt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIASpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofPlant Foods for Human Nutrition-
dc.rightsAcesso Restritopt_BR
dc.subjectNucleotídeos cíclicospt_BR
dc.subjectComputadores molecularespt_BR
dc.subject.otherNucleotídeos cíclicospt_BR
dc.subject.otherComputadores molecularespt_BR
dc.subject.otherPlaquetas (Sangue)pt_BR
dc.subject.otherHibiscopt_BR
dc.titleEffect of different extraction methods on anthocyanin content in hibiscus sabdariffa L. and their antiplatelet and vasorelaxant propertiespt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://pubmed.ncbi.nlm.nih.gov/37120677/pt_BR
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