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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/40516
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dc.creatorLetícia Rocha Guidipt_BR
dc.creatorFlávio Alves Santospt_BR
dc.creatorAna Cláudia dos Santos Rossi Ribeiropt_BR
dc.creatorChristian Fernandespt_BR
dc.creatorLuiza Helena Meller da Silvapt_BR
dc.creatorMaria Beatriz de Abreu Glóriapt_BR
dc.date.accessioned2022-03-28T21:05:36Z-
dc.date.available2022-03-28T21:05:36Z-
dc.date.issued2017-01-15-
dc.citation.volume163pt_BR
dc.citation.spage85pt_BR
dc.citation.epage93pt_BR
dc.identifier.doi10.1016/j.talanta.2016.10.089pt_BR
dc.identifier.issn00399140pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/40516-
dc.description.resumoThe objective of this study was to develop and validate a fast, sensitive and simple liquid chromatography–electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method for the screening of six classes of antibiotics (aminoglycosides, beta-lactams, macrolides, quinolones, sulfonamides and tetracyclines) in fish. Samples were extracted with trichloroacetic acid. LC separation was achieved on a Zorbax Eclipse XDB C18 column and gradient elution using 0.1% heptafluorobutyric acid in water and acetonitrile as mobile phase. Analysis was carried out in multiple reaction monitoring mode via electrospray interface operated in the positive ionization mode, with sulfaphenazole as internal standard. The method was suitable for routine screening purposes of 40 antibiotics, according to EC Guidelines for the Validation of Screening Methods for Residues of Veterinary Medicines, taking into consideration threshold value, cut-off factor, detection capability, limit of detection, sensitivity and specificity. Real fish samples (n=193) from aquaculture were analyzed and 15% were positive for enrofloxacin (quinolone), one of them at a higher concentration than the level of interest (50 µg kg−1), suggesting possible contamination or illegal use of that antibiotic.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE ALIMENTOSpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOSpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofTalanta (oxford)pt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectAminoglycosidept_BR
dc.subjectBeta-Lactampt_BR
dc.subjectMacrolidept_BR
dc.subjectQuinolonept_BR
dc.subjectSulfonamidept_BR
dc.subjectAquaculturept_BR
dc.subject.otherFarmáciapt_BR
dc.subject.otherPeixespt_BR
dc.subject.otherAntibióticospt_BR
dc.titleA simple, fast and sensitive screening LC-ESI-MS/MS method for antibiotics in fishpt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.sciencedirect.com/science/article/pii/S0039914016308451pt_BR
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