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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/41308
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dc.creatorMarliete Carvalho Costapt_BR
dc.creatorLays Murta Matapt_BR
dc.creatorNoelly de Queiroz Ribeiropt_BR
dc.creatorAnderson Philip Nonato Santospt_BR
dc.creatorLorena Vivien Neves Oliveirapt_BR
dc.creatorRaquel Virgínia Rocha Vilelapt_BR
dc.creatorValbert Nascimento Cardosopt_BR
dc.creatorSimone Odília Antunes Fernandespt_BR
dc.creatorDaniel Assis Santospt_BR
dc.date.accessioned2022-05-03T01:20:43Z-
dc.date.available2022-05-03T01:20:43Z-
dc.date.issued2018-06-
dc.citation.volume56pt_BR
dc.citation.issue4pt_BR
dc.citation.spage479pt_BR
dc.citation.epage484pt_BR
dc.identifier.doi10.1093/mmy/myx060pt_BR
dc.identifier.issn1369-3786pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/41308-
dc.description.resumoCryptococcus gattii is one of the etiologic agents of cryptococcosis, a systemic mycosis that occurs in healthy and immunosuppressed humans and animals worldwide. Primary pulmonary infection caused by C. gattii is usually followed by fungal dissemination to the central nervous system, resulting in high mortality rates. In this context, animal models of cryptococcosis are useful in the study of fungal pathogenesis and host response against the pathogen, and for testing novel therapeutic options. The most frequently applied method to study fungal dissemination from the lungs to other organs is by culturing tissues, which is not accurate for the detection and quantification of fungal load at early stages of the infection. To overcome this problem, the purpose of this study was to develop a new method for the quantification of Cryptococcus dissemination. One C. gattii strain was efficiently radiolabeled with technetium-99m (99mTc), without affecting viability of the cells. Further, the 99mTc-C. gattii (111 MBq) strain was used to infect mice by intratracheal and intravenous route for biodistribution studies. 99mTc-C. gattii was successfully used in detection of the yeast in the brain of mice 6 hours postinoculation, while the detection using colony forming units was possible only 24 hours postinfection. Our results provided an alternative method that could be applied in further investigations regarding the efficacy of antifungals, fungal virulence, and host-pathogen interactions.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICASpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofMedical Mycologypt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectAnimal modelpt_BR
dc.subjectFungal disseminationpt_BR
dc.subjectRadiolabelingpt_BR
dc.subjectCryptococcus gattiipt_BR
dc.subjectTechnetium-99mpt_BR
dc.subject.otherMicosept_BR
dc.subject.otherCryptococcus gattiipt_BR
dc.titleA new method for studying cryptococcosis in a murine model using 99mTc-Cryptococcus gattiipt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://academic.oup.com/mmy/article-lookup/doi/10.1093/mmy/myx060pt_BR
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