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http://hdl.handle.net/1843/41308
Full metadata record
DC Field | Value | Language |
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dc.creator | Marliete Carvalho Costa | pt_BR |
dc.creator | Lays Murta Mata | pt_BR |
dc.creator | Noelly de Queiroz Ribeiro | pt_BR |
dc.creator | Anderson Philip Nonato Santos | pt_BR |
dc.creator | Lorena Vivien Neves Oliveira | pt_BR |
dc.creator | Raquel Virgínia Rocha Vilela | pt_BR |
dc.creator | Valbert Nascimento Cardoso | pt_BR |
dc.creator | Simone Odília Antunes Fernandes | pt_BR |
dc.creator | Daniel Assis Santos | pt_BR |
dc.date.accessioned | 2022-05-03T01:20:43Z | - |
dc.date.available | 2022-05-03T01:20:43Z | - |
dc.date.issued | 2018-06 | - |
dc.citation.volume | 56 | pt_BR |
dc.citation.issue | 4 | pt_BR |
dc.citation.spage | 479 | pt_BR |
dc.citation.epage | 484 | pt_BR |
dc.identifier.doi | 10.1093/mmy/myx060 | pt_BR |
dc.identifier.issn | 1369-3786 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/1843/41308 | - |
dc.description.resumo | Cryptococcus gattii is one of the etiologic agents of cryptococcosis, a systemic mycosis that occurs in healthy and immunosuppressed humans and animals worldwide. Primary pulmonary infection caused by C. gattii is usually followed by fungal dissemination to the central nervous system, resulting in high mortality rates. In this context, animal models of cryptococcosis are useful in the study of fungal pathogenesis and host response against the pathogen, and for testing novel therapeutic options. The most frequently applied method to study fungal dissemination from the lungs to other organs is by culturing tissues, which is not accurate for the detection and quantification of fungal load at early stages of the infection. To overcome this problem, the purpose of this study was to develop a new method for the quantification of Cryptococcus dissemination. One C. gattii strain was efficiently radiolabeled with technetium-99m (99mTc), without affecting viability of the cells. Further, the 99mTc-C. gattii (111 MBq) strain was used to infect mice by intratracheal and intravenous route for biodistribution studies. 99mTc-C. gattii was successfully used in detection of the yeast in the brain of mice 6 hours postinoculation, while the detection using colony forming units was possible only 24 hours postinfection. Our results provided an alternative method that could be applied in further investigations regarding the efficacy of antifungals, fungal virulence, and host-pathogen interactions. | pt_BR |
dc.description.sponsorship | CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico | pt_BR |
dc.description.sponsorship | FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais | pt_BR |
dc.description.sponsorship | CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior | pt_BR |
dc.format.mimetype | pt_BR | |
dc.language | eng | pt_BR |
dc.publisher | Universidade Federal de Minas Gerais | pt_BR |
dc.publisher.country | Brasil | pt_BR |
dc.publisher.department | FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS | pt_BR |
dc.publisher.initials | UFMG | pt_BR |
dc.relation.ispartof | Medical Mycology | pt_BR |
dc.rights | Acesso Aberto | pt_BR |
dc.subject | Animal model | pt_BR |
dc.subject | Fungal dissemination | pt_BR |
dc.subject | Radiolabeling | pt_BR |
dc.subject | Cryptococcus gattii | pt_BR |
dc.subject | Technetium-99m | pt_BR |
dc.subject.other | Micose | pt_BR |
dc.subject.other | Cryptococcus gattii | pt_BR |
dc.title | A new method for studying cryptococcosis in a murine model using 99mTc-Cryptococcus gattii | pt_BR |
dc.type | Artigo de Periódico | pt_BR |
dc.url.externa | https://academic.oup.com/mmy/article-lookup/doi/10.1093/mmy/myx060 | pt_BR |
Appears in Collections: | Artigo de Periódico |
Files in This Item:
File | Description | Size | Format | |
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A new method for studying cryptococcosis in a murine model using 99mTc-Cryptococcus gattii.pdf | 448.31 kB | Adobe PDF | View/Open |
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