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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/41491
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dc.creatorCamila Maria de Sousa Lacerdapt_BR
dc.creatorIêda Mendes Ferreirapt_BR
dc.creatorSara Roberta dos Santospt_BR
dc.creatorAndré Luís Branco de Barrospt_BR
dc.creatorSimone Odília Fernandespt_BR
dc.creatorValbert Nascimento Cardosopt_BR
dc.creatorAntero Silva Ribeiro de Andradept_BR
dc.date.accessioned2022-05-09T20:34:15Z-
dc.date.available2022-05-09T20:34:15Z-
dc.date.issued2017-03-
dc.citation.volume46pt_BR
dc.citation.spage19pt_BR
dc.citation.epage24pt_BR
dc.identifier.doi10.1016/j.nucmedbio.2016.11.008pt_BR
dc.identifier.issn0969-8051pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/41491-
dc.description.resumoIntroduction Acid nucleic aptamers are RNA or DNA oligonucleotides capable of binding to a target molecule with high affinity and selectivity. These molecules are promising tools in nuclear medicine. Many aptamers have been used as targeting molecule of radiopharmaceuticals in preclinical studies. (1 → 3)-β-D-glucans are the main structural cell wall components of fungi and some bacteria. In the present study two radiolabeled (1 → 3)-β-D-glucan aptamers (seq6 and seq30) were evaluated to identity infectious foci caused by fungal or bacterial cells. Methods Aptamer labeling with 99mTc was performed by the direct method and biodistribution studies were accomplished in Swiss mice (n = 6) infected in the right thigh muscle with Staphylococcus aureus or Candida albicans. A 99mTc radiolabeled library consisting of oligonucleotides with random sequences was used as control. Results There was a higher uptake of 99mTc radiolabeled aptamers in the infected thigh than in the left thigh muscle (non-infected) in the S. aureus infected animals. The target/non-target ratios were 3.17 ± 0.22 for seq6 and 2.66 ± 0.10 for seq30. These ratios were statistically higher than the value (1.54 ± 0.05) found for the radiolabeled library (control). With regard to biodistribution, no statistical difference was verified between aptamers and control uptakes in the infection foci in the C. albicans infected animals. The target/non-target ratios were 1.53 ± 0.03, 1.64 ± 0.12 and 1.08 ± 0.02 for radiolabeled library, seq6 and seq30, respectively. Scintigraphic imaging of infected foci using radiolabeled aptamers was possible only for S. aureus infected mice. Conclusions Seq6 and seq30 aptamers proved to be inefficient for diagnosis of C. albicans infection. Nevertheless, their applicability for diagnosis of S. aureus and other bacterial infections by scintigraphy should be further explored.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipOutra Agênciapt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE ALIMENTOSpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICASpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofNuclear Medicine and Biologypt_BR
dc.rightsAcesso Restritopt_BR
dc.subjectRadiopharmaceuticalpt_BR
dc.subjectBiodistributionpt_BR
dc.subjectTechnetium-99 mpt_BR
dc.subject(1 → 3)-β-D-glucanspt_BR
dc.subjectAptamerpt_BR
dc.subjectInfection diagnosispt_BR
dc.subject.otherRadiofármacopt_BR
dc.subject.otherBiodistribuiçãopt_BR
dc.subject.otherDiagnóstico de infecçõespt_BR
dc.title(1 → 3)-β-D-glucan aptamers labeled with technetium-99 m: Biodistribution and imaging in experimental models of bacterial and fungal infectionpt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.sciencedirect.com/science/article/pii/S0969805116301688?via%3Dihubpt_BR
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