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Type:	Artigo de Periódico
Title:	Steric mass action model for lactoferrin adsorption in cryogel with immobilized copper ions
Authors:	Bruna Mara Aparecida de Carvalho Willer Ferreira Silva Júnior Lorendane Millena Carvalho Luis Antonio Minim Gleidson Giordano Pinto de Carvalho
Abstract:	Parameters of equilibrium adsorption obtained from experiments using immobilized metal affinity chromatography (IMAC) were used to evaluate the applicability of the steric mass-action (SMA) model to describe the adsorption of lactoferrin to cryogel resin under different conditions. The adsorption of lactoferrin on continuous supermacroporous cryogel with immobilized Cu2+ ions was evaluated in batch adsorption experiments at different pH (6-8) and temperature (293-313 K) values. Estimated values of the equilibrium constant (K) and characteristic number of binding sites (n) showed that these parameters decreased with increasing ionic strength, pH and temperature, while the nonlinear parameter, the steric factor (σ), increased with increasing ionic strength and temperature. Expressions correlating these parameters with pH, ionic strength and temperature were then derived.
Subject:	Cromatografia de afinidade Proteínas Glicoproteinas
language:	eng
metadata.dc.publisher.country:	Brasil
Publisher:	Universidade Federal de Minas Gerais
Publisher Initials:	UFMG
metadata.dc.publisher.department:	ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
Rights:	Acesso Aberto
metadata.dc.identifier.doi:	https://doi.org/10.1590/0104-6632.20160331s20140112
URI:	http://hdl.handle.net/1843/42620
Issue Date:	2016
metadata.dc.url.externa:	https://www.scielo.br/j/bjce/a/VvnCb5bpsSsgPSvrxmnpYgh/?lang=en
metadata.dc.relation.ispartof:	Brazilian Journal of Chemical Engineering
Appears in Collections:	Artigo de Periódico

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