An accurate identification of Pythium insidiosum complex using MALDI-TOF, requires representative spectra of each of its four phylogenetic clusters

Leonel Mendoza; Raquel Virgínia Rocha Vilela; Rinosh Mani

Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/46256

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DC Field	Value	Language
dc.creator	Leonel Mendoza	pt_BR
dc.creator	Raquel Virgínia Rocha Vilela	pt_BR
dc.creator	Rinosh Mani	pt_BR
dc.date.accessioned	2022-10-17T15:23:11Z	-
dc.date.available	2022-10-17T15:23:11Z	-
dc.date.issued	2019-10-01	-
dc.citation.volume	87	pt_BR
dc.citation.spage	193	pt_BR
dc.identifier.doi	https://doi.org/10.1016/j.ijid.2019.07.025	pt_BR
dc.identifier.issn	1201-9712	pt_BR
dc.identifier.uri	http://hdl.handle.net/1843/46256	-
dc.description.abstract	Em 15 de junho de 2019, um relato de caso apareceu na edição online do International Journal of Infectious Diseases (IJID) por Bernheim et al., 2019. No relatório, os autores descreveram o manejo de um caso de ceratite causada por Pythium insidiosum em um homem da Espanha. O diagnóstico foi feito por meio de metodologias moleculares, mas nenhuma sequência de DNA foi depositada no GenBank. O isolamento de “um grande micélio” em Brain-Heart-Infusion (BHI) foi mencionado, mas o isolado não foi depositado em uma coleção de cultura. Embora uma biópsia ocular usando primers pan-fúngicos tenha identificado o organismo no tecido biopsiado como P. insidiosum, não havia uma descrição de como o isolado recuperado do BHI foi finalmente identificado como P. insidiosum.	pt_BR
dc.description.resumo	On June 15 2019, a case report appeared in the online edition of International Journal of Infectious Diseases (IJID) by Bernheim et al., 2019. In the report, the authors described the management of a keratitis case caused by Pythium insidiosum in a man from Spain. The diagnosis was made through molecular methodologies, but no DNA sequences were deposited at GenBank. The isolation of “a large mycelium” on Brain-Heart-Infusion (BHI) was mentioned, but the isolate was not deposited at a culture collection. Although an eye biopsy using pan-fungal primers identified the organism in the biopsied tissue as P. insidiosum, there was not a description on how the isolate recovered from BHI was finally identified as P. insidiosum.	pt_BR
dc.format.mimetype	pdf	pt_BR
dc.language	eng	pt_BR
dc.publisher	Universidade Federal de Minas Gerais	pt_BR
dc.publisher.country	Brasil	pt_BR
dc.publisher.department	FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS	pt_BR
dc.publisher.initials	UFMG	pt_BR
dc.relation.ispartof	International Journal of Infectious Diseases	pt_BR
dc.rights	Acesso Aberto	pt_BR
dc.subject	Keratitis	pt_BR
dc.subject	Pythium	pt_BR
dc.subject	Molecular phylogeny	pt_BR
dc.subject.other	Ceratite	pt_BR
dc.subject.other	Pythium	pt_BR
dc.subject.other	Filogenia	pt_BR
dc.title	An accurate identification of Pythium insidiosum complex using MALDI-TOF, requires representative spectra of each of its four phylogenetic clusters	pt_BR
dc.type	Artigo de Periódico	pt_BR
dc.url.externa	https://www.ijidonline.com/article/S1201-9712(19)30310-8/fulltext#relatedArticles	pt_BR
Appears in Collections:	Artigo de Periódico

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