Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/51266
Type: Artigo de Periódico
Title: Miniaturized methodologies for determining the total phenol and flavonoid concentrations and the antioxidant activity
Authors: Ane Patrícia Cacique
Érica Soares Barbosa
Gevany Paulino de Pinho
Flaviano Oliveira Silvério
Abstract: The present study describes the miniaturization of the conventional methodologies for determining the total phenol and flavonoid concentrations and the antioxidant activities using DPPH, ABTS, and ferric reducing antioxidant power (FRAP) methods. The amount of reagents used was reduced 25, 5.0, 3.6, 3.0, and 3.3 times relative to the conventional methodologies, representing a 67–96% reduction in the analysis costs. The miniaturized and conventional methodologies presented similar linearity in the same concentration range, with the determination and correlation coefficients greater than 0.99. The accuracy of the methodologies was evidenced by the relative standard deviations, which remained below 3%. The 38 samples comprising alcoholic beverages, teas, spices, jams, energy drinks, honey products, and grape derivatives analyzed using the two methodologies (miniaturized and conventional) presented similar average values. Therefore, the miniaturized methodology is proposed as an efficient, safe, and economical alternative to the conventional methods for determining the total phenol and flavonoid concentrations and the antioxidant activities using DPPH, ABTS, and FRAP methods in various matrices.
Subject: Fenóis
Flavonóides
Espectrofotometria
language: eng
metadata.dc.publisher.country: Brasil
Publisher: Universidade Federal de Minas Gerais
Publisher Initials: UFMG
metadata.dc.publisher.department: ICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
Rights: Acesso Restrito
metadata.dc.identifier.doi: https://doi.org/10.1007/s12161-020-01934-w
URI: http://hdl.handle.net/1843/51266
Issue Date: 15-Jan-2021
metadata.dc.url.externa: https://link.springer.com/article/10.1007/s12161-020-01934-w
metadata.dc.relation.ispartof: Food Analytical Methods
Appears in Collections:Artigo de Periódico

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