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Use este identificador para citar ou linkar para este item: http://hdl.handle.net/1843/52327
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Campo DCValorIdioma
dc.creatorNaialy Fernandes Araújo Reispt_BR
dc.creatorLuiz Felipe Gomes da Silvapt_BR
dc.creatorMateus Araújo Castro e Souzapt_BR
dc.creatorSílvia Ligório Fialhopt_BR
dc.creatorCarolina Paula de Souza Moreirapt_BR
dc.creatorMarcus Vinicius de Oliveira Andradept_BR
dc.creatorWashington Xavier de Paulapt_BR
dc.creatorMaria Beatriz de Abreu Glóriapt_BR
dc.creatorGerson Antônio Pianettipt_BR
dc.creatorChristian Fernandespt_BR
dc.date.accessioned2023-04-20T20:46:27Z-
dc.date.available2023-04-20T20:46:27Z-
dc.date.issued2021-01-
dc.citation.volume59pt_BR
dc.citation.issue1pt_BR
dc.citation.spage30pt_BR
dc.citation.epage39pt_BR
dc.identifier.doihttps://doi.org/10.1093/chromsci/bmaa073pt_BR
dc.identifier.issn00219665pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/52327-
dc.description.resumoOne of the highest incidences of illegal drug products is related to phosphodiesterase-5 inhibitors, used in treatment of erectile dysfunction, including those containing sildenafil citrate and tadalafil. In this context, comprehensive evaluation of the quality of genuine and illegal medicines was performed. A simple and rapid ultra-high performance liquid chromatography (UHPLC-UV) method to quantify sildenafil and tadalafil in the presence of six degradation products was developed and validated. Sildenafil and tadalafil were submitted to forced degradation. The separation was carried out on a Kinetex C18 (50 × 2.1 mm; 1.7 μm) column with mobile phase composed of acetonitrile and aqueous triethylamine solution. The calibration curves were linear in the range of 14–126 μg mL−1 for sildenafil citrate and 4–36 μg mL−1 for tadalafil and the method proved to be selective, precise, accurate and robust. Sildenafil degraded in oxidative media, whereas tadalafil degraded in acidic, alkaline and oxidative environment. The chemical structures and the mechanisms for the formation of the main degradation products were proposed by UHPLC coupled to tandem mass spectrometry. The UHPLC-UV method was applied in the pharmaceutical analysis of genuine and seized medicines. Some of them did not meet quality standards, mainly due to contents below specifications and the large variation on contents between units within a batch.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE ALIMENTOSpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOSpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of Chromatographic Science-
dc.rightsAcesso Abertopt_BR
dc.subjectSeizure medicinespt_BR
dc.subjectSildenafilpt_BR
dc.subjectTadalafilpt_BR
dc.subjectForced degradationpt_BR
dc.subjectUHPLC-MS/MSpt_BR
dc.subject.otherFarmáciapt_BR
dc.subject.otherMedicamentospt_BR
dc.subject.otherQualidade de medicamentospt_BR
dc.subject.otherCromatografia líquidapt_BR
dc.titleUHPLC for quality evaluation of genuine and illegal medicines containing sildenafil citrate and tadalafilpt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://academic.oup.com/chromsci/article/59/1/30/5940794pt_BR
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