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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/52440
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dc.creatorLetícia Ferrari Crocomopt_BR
dc.creatorFederica Ariupt_BR
dc.creatorLuisa Bogliolopt_BR
dc.creatorDaniela Bebberept_BR
dc.creatorSergio Leddapt_BR
dc.creatorSony Dimas Bicudopt_BR
dc.date.accessioned2023-04-24T21:48:51Z-
dc.date.available2023-04-24T21:48:51Z-
dc.date.issued2016-
dc.citation.volume51pt_BR
dc.citation.issue2pt_BR
dc.citation.spage276pt_BR
dc.citation.epage281pt_BR
dc.identifier.issn0936-6768pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/52440-
dc.description.resumoThe efficiency of in vitro sheep embryo production is still low compared to that observed in vivo and in other species. In this context, meiotic inhibition strategies emerged as a promising alternative to improve this biotechnology. So, this study aimed to evaluate, for the first time, the effects of roscovitine on in vitro maturation of sheep oocytes and their subsequent embryo development. For this, cumulus-oocyte complexes (COCs) were cultured for 6 h in the presence (Rosco) or absence (Control) of 75 μm roscovitine and, subsequently, in vitro matured (IVM) for 18 h with gonadotropins. At 0 (Immature), 6 and 24 h of culture, the nuclear status of oocytes was evaluated by Hoechst staining. Embryo cleavage and blastocyst formation were recorded 30 h after in vitro fertilization and on day 7 of culture, respectively. Blastocyst quality was evaluated by differential staining. At 6 h, the GV rate in the Rosco treatment (93.8%) was similar to that observed in the Immature oocytes (94.9%) and significantly higher compared to Control (41.3%). After IVM for 18 h, a high and similar proportion of oocytes from Rosco (93.6%) and Control (88.4%) reached the MII stage. In both treatments, approximately 70% of oocytes cleaved and 50% of them developed up to blastocyst. The mean percentage of blastocyst cells, embryoblast, trophoblast and pyknosis did also not differ between Control and Rosco. In conclusion, roscovitine, at the studied experimental conditions, was efficient to reversibly inhibit the meiosis of adult sheep oocytes without detrimental effect on development and quality of the in vitro produced embryos.pt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIASpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofReproduction in Domestic Animals-
dc.rightsAcesso Restritopt_BR
dc.subjectOvelhapt_BR
dc.subjectBiotecnologiapt_BR
dc.subjectFertilização in vitropt_BR
dc.subject.otherOvelhapt_BR
dc.subject.otherBiotecnologiapt_BR
dc.subject.otherFertilização in vitropt_BR
dc.titleIn vitro developmental competence of adult sheep oocytes treated with roscovitinept_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://pubmed.ncbi.nlm.nih.gov/26890275/pt_BR
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