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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/52488
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dc.creatorDaniela Martins Paschoalpt_BR
dc.creatorFernanda da Cruz Landim-Alvarengapt_BR
dc.creatorMateus José Sudanopt_BR
dc.creatorKátia Regina Lancellotti Schwarzpt_BR
dc.creatorRosiára Rosário Dias Mazieropt_BR
dc.creatorMidyan Daroz Guastalipt_BR
dc.creatorLetícia Ferrari Crocomopt_BR
dc.creatorLuis Carlos Oña Magalhãespt_BR
dc.creatorAlício Martins Júniorpt_BR
dc.creatorClaudia Lima Verde Lealpt_BR
dc.date.accessioned2023-04-25T21:55:15Z-
dc.date.available2023-04-25T21:55:15Z-
dc.date.issued2017-
dc.citation.volume87pt_BR
dc.citation.spage108pt_BR
dc.citation.epage114pt_BR
dc.identifier.doihttps://doi.org/10.1016/j.theriogenology.2016.08.011pt_BR
dc.identifier.issn0093-691Xpt_BR
dc.identifier.urihttp://hdl.handle.net/1843/52488-
dc.description.resumoThe presence of fetal calf serum in culture medium influences embryo quality, causing a reduction in postcryopreservation survival. Forskolin has been used to induce lipolysis and increase cryotolerance, functioning as an activator of adenylate cyclase and elevating cAMP levels. In the present experiment, bovine zygotes were cultured in synthetic oviduct fluid with amino acid plus 2.5% fetal calf serum for 6 days, when forskolin was added in three concentrations: 2.5, 5, and 10 mM. Treatment with forskolin lasted for 24 hours. Blastocyst formation rate, quantification of lipid granules, total cell numbers, and apoptosis rate were evaluated. In a second assessment, embryos were vitrified, and warming, re-expansion rate, total cell numbers, and apoptosis rate were also evaluated. There was no difference due to forskolin in blastocyst formation or re-expansion rates after vitrification. However, lipid measurements were lower (control: 136.8 and F 2.5 mM: 128.5; P < 0.05), and number of cells per embryo higher (control: 140.1 and F 2.5 mM: 173.5; P < 0.05) than controls for 2.5 mM forskolin but not for higher forskolin concentrations. The number of intact cells per embryo was higher, and the rate of apoptosis was lower in fresh than in vitrified embryos (number of cells of warmed embryos, control: 104.1, F 2.5 mM: 101.3, F 5 mM: 115.4, F 10 mM: 95.1; apoptotic of fresh cells, control: 12.1%, F 2.5 mM: 16.7%, F 5 mM: 11.1%, F 10 mM: 14.2%; and apoptotic warmed embryos, control: 22.3%, F 2.5 mM: 37.3%, F 5 mM: 33.2%, F 10 mM: 30.3%; P < 0.05). It was concluded that forskolin is an effective lipolytic agent even at low concentrations, leading to formation of blastocysts with a comparatively larger number of cells.pt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIASpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofTheriogenology-
dc.rightsAcesso Restritopt_BR
dc.subjectApoptosispt_BR
dc.subjectBlastocystpt_BR
dc.subjectCryopreservationpt_BR
dc.subjectForskolinpt_BR
dc.subjectLipidpt_BR
dc.subjectVitrificationpt_BR
dc.subject.otherLipidiospt_BR
dc.subject.otherApoptosept_BR
dc.subject.otherBlastocistopt_BR
dc.titleCell apoptosis and lipid content of in vitro-produced, vitrified bovine embryos treated with forskolinpt_BR
dc.typeArtigo de Periódicopt_BR
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