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http://hdl.handle.net/1843/54168| Type: | Artigo de Periódico |
| Title: | Starch combined with sucrose provokes greater root dentine demineralization than sucrose alone |
| Authors: | Samilly Evangelista Souza Aline Araújo Sampaio Altair Antoninha del Bel Cury Yuri Wanderley Cavalcanti Antônio Pedro Ricomini Filho Jaime Aparecido Cury |
| Abstract: | Since there is no consensus about whether starch increases the cariogenic potential of sucrose, we used a validated 3-species biofilm model to evaluate if starch combined with sucrose provokes higher root dentine demineralization than sucrose alone. Biofilms (n = 18) composed by Streptococcus mutans (the most cariogenic bacteria), Actinomces naeslundii (which has amylolytic activity), and Streptococcus gordonii (which binds salivary amylase) were formed on root dentine slabs under exposure 8 ×/day to one of the following treatments: 0.9% NaCl, 1% starch, 10% sucrose, or a combination of 1% starch and 10% sucrose. Before each treatment, biofilms were pretreated with human whole saliva for 1 min. The pH of the culture medium was measured daily as an indicator of biofilm acidogenicity. After 96 h of growth, the biofilms were collected, and the biomass, bacteria viability, and polysaccharides were analyzed. Dentine demineralization was assessed by surface hardness loss (% SHL). Biofilm bioarchitecture was analyzed using confocal laser scanning microscopy. Treatment with a starch and sucrose combination provoked higher (p = 0.01) dentine demineralization than sucrose alone (% SHL = 53.2 ± 7.0 vs. 43.2 ± 8.7). This was supported by lower pH values (p = 0.007) of the culture medium after daily exposure to the starch and sucrose combination compared with sucrose (4.89 ± 0.29 vs. 5.19 ± 0.32). Microbiological and biochemical findings did not differ between biofilms treated with the combination of starch and sucrose and sucrose alone (p > 0.05). Our findings give support to the hypothesis that a starch and sucrose combination is more cariogenic for root dentine than sucrose alone |
| Subject: | Biofilms Demineralization Dentin Starch Sucrose Bacteria Biomass Hydrogen-íon concentration |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAO - DEPARTAMENTO DE CLÍNICA |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | https://doi.org/10.1159/000486427 |
| URI: | http://hdl.handle.net/1843/54168 |
| Issue Date: | 2018 |
| metadata.dc.url.externa: | https://karger.com/cre/article/52/4/323/85685/Starch-Combined-with-Sucrose-Provokes-Greater-Root |
| metadata.dc.relation.ispartof: | Caries Research |
| Appears in Collections: | Artigo de Periódico |
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