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Use este identificador para citar ou linkar para este item: http://hdl.handle.net/1843/54940
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Campo DCValorIdioma
dc.creatorRenato Sathler-avelarpt_BR
dc.creatorOlindo Assis Martins-filhopt_BR
dc.creatorEdward j. Dickpt_BR
dc.creatorGene b. Hubbardpt_BR
dc.creatorJane f. Vandebergpt_BR
dc.creatorJohn l. Vandebergpt_BR
dc.creatorDanielle Marchetti Vitelli Avelarpt_BR
dc.creatorArmanda Moreira Mattoso-barbosapt_BR
dc.creatorMarcelo Perdigão-de-oliveirapt_BR
dc.creatorRonaldo Peres Costapt_BR
dc.creatorSilvana Maria Elói Santospt_BR
dc.creatorMatheus de Souza Gomespt_BR
dc.creatorLaurence Rodrigues do Amaralpt_BR
dc.creatorAndréa Teixeira-carvalhopt_BR
dc.date.accessioned2023-06-14T20:24:14Z-
dc.date.available2023-06-14T20:24:14Z-
dc.date.issued2016-01-25-
dc.citation.volume10pt_BR
dc.citation.issue1pt_BR
dc.citation.spage1pt_BR
dc.citation.epage16pt_BR
dc.identifier.doi10.1371/journal.pntd.0004302pt_BR
dc.identifier.issn19352735pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/54940-
dc.description.resumoCynomolgus macaques (Macaca fascicularis) represent a feasible model for research on Chagas disease since natural T. cruzi infection in these primates leads to clinical outcomes similar to those observed in humans. However, it is still unknown whether these clinical similarities are accompanied by equivalent immunological characteristics in the two species.We have performed a detailed immunophenotypic analysis of circulating leukocytes together with systems biology approaches from 15 cynomolgus macaques naturally. Methods and Findings Our data established that CH displayed increased expression of CD32+ and CD56+ in monocytes and enhanced frequency of NK Granzyme A+ cells as compared to non-infected infected with T. cruzi (CH) presenting the chronic phase of Chagas disease to identify biomarkers that might be useful for clinical investigations controls (NI). Moreover, higher expression of CD54 and HLA-DR by T-cells, especially within the CD8+ subset, was the hallmark of CH. A high level of expression of Granzyme A and Perforin underscored the enhanced cytotoxicity-linked pattern of CD8+ T-lymphocytes from CH. Increased frequency of B-cells with up-regulated expression of Fc-γRII was also observed in CH. Complex and imbricate biomarker networks demonstrated that CH showed a shift towards cross-talk among cells of the adaptive immune system. Systems biology analysis further established monocytes and NK-cell phenotypes and the T-cell activation status, along with the Granzyme A expression by CD8+ T-cells, as the most reliable biomarkers of potential use for clinical applications Conclusions: Altogether, these findings demonstrated that the similarities in phenotypic features of circulating leukocytes observed in cynomolgus macaques and humans infected with T. cruzi further supports the use of these monkeys in preclinical toxicology and pharmacology studiesapplied to development and testing of new drugs for Chagas disease.pt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentMED - DEPARTAMENTO DE PROPEDÊUTICA COMPLEMENTARpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofPLOS Neglected Tropical Diseases-
dc.rightsAcesso Abertopt_BR
dc.subjectDoença de Chagaspt_BR
dc.subjectMacaca fascicularispt_BR
dc.subjectGranzimaspt_BR
dc.subject.otherDoença de Chagaspt_BR
dc.subject.otherMacaca fascicularispt_BR
dc.subject.otherGranzimaspt_BR
dc.titlePhenotypic features of circulating leukocytes from non-human primates naturally infected with trypanosoma cruzi resemble the major immunological findings observed in human chagas diseasept_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://doi:10.1371/journal.pntd.0004302pt_BR
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