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http://hdl.handle.net/1843/58652| Tipo: | Artigo de Periódico |
| Título: | Angiotensin-(1-7) and Alamandine Promote Anti-inflammatory Response in Macrophages In Vitro and In Vivo |
| Autor(es): | Melissa de Carvalho Santuchi Ricardo Gonçalves Mauro Martins Teixeira Lirlândia Pires Sousa Robson Augusto Souza Dos Santos Rafaela Fernandes da Silva Miriane Fernandes Dutra Juliana Priscila Vago Kátia Maciel Lima Izabela Galvão Fernando Pedro de Souza-neto Mario Morais e Silva Aline Cristina Oliveira Flávia Carvalho Bittencourt de Oliveira |
| Resumo: | The renin-angiotensin system (RAS) peptides play an important role in inflammation. Resolution of inflammation contributes to restore tissue homeostasis, and it is characterized by neutrophil apoptosis and their subsequent removal by macrophages, which are remarkable plastic cells involved in the pathophysiology of diverse inflammatory diseases. However, the effects of RAS peptides on different macrophage phenotypes are still emerging. Here, we evaluated the effects of angiotensin-(1-7) (Ang-(1-7)) and the most novel RAS peptide, alamandine, on resting (M0), proinflammatory M(LPS+IFN-γ), and anti-inflammatory M(IL-4) macrophage phenotypes in vitro, as well as on specific immune cell populations and macrophage subsets into the pleural cavity of LPS-induced pleurisy in mice. Our results showed that Ang-(1-7) and alamandine, through Mas and MrgD receptors, respectively, do not affect M0 macrophages but reduce the proinflammatory TNF-α, CCL2, and IL-1β transcript expression levels in LPS+IFN-γ-stimulated macrophages. Therapeutic administration of these peptides in LPS-induced inflammation in mice decreased the number of neutrophils and M1 (F4/80lowGr1+CD11bmed) macrophage frequency without affecting the other investigated macrophage subsets. Our data suggested that both Ang-(1-7) and alamandine, through their respective receptors Mas and MrgD, promote an anti-inflammatory reprogramming of M(LPS+IFN-γ)/M1 macrophages under inflammatory circumstances and potentiate the reprogramming induced by IL-4. In conclusion, our work sheds light on the emerging proresolving properties of Ang-(1-7) and alamandine, opening new avenues for the treatment of inflammatory diseases. |
| Assunto: | Infeccções Macrófagos |
| Idioma: | eng |
| País: | Brasil |
| Editor: | Universidade Federal de Minas Gerais |
| Sigla da Instituição: | UFMG |
| Departamento: | FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS ICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA ICB - DEPARTAMENTO DE FARMACOLOGIA ICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICA ICB - DEPARTAMENTO DE PATOLOGIA |
| Tipo de Acesso: | Acesso Aberto |
| Identificador DOI: | https://doi.org/10.1155/2019/2401081 |
| URI: | http://hdl.handle.net/1843/58652 |
| Data do documento: | 21-Fev-2019 |
| metadata.dc.url.externa: | https://www.hindawi.com/journals/mi/2019/2401081/#copyright |
| metadata.dc.relation.ispartof: | Mediators of Inflammation |
| Aparece nas coleções: | Artigo de Periódico |
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| Arquivo | Descrição | Tamanho | Formato | |
|---|---|---|---|---|
| Angiotensin-(1-7) and Alamandine Promote Anti-inflammatory Response in Macrophages In Vitro and In Vivo.pdf | 4.8 MB | Adobe PDF | Visualizar/Abrir |
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