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Use este identificador para citar o ir al link de este elemento: http://hdl.handle.net/1843/60284
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Campo DCValorIdioma
dc.creatorAnny Carolline Silva Oliveirapt_BR
dc.creatorHélida Monteiro de Andradept_BR
dc.creatorLuciana de Oliveira Andradept_BR
dc.creatorLuisa Rezendept_BR
dc.creatorVladimir Gorshkovpt_BR
dc.creatorMarcella Nunes Melo-Bragapt_BR
dc.creatorWeslley Fernandes-Bragapt_BR
dc.creatorJorge Luís de Melo Guadalupept_BR
dc.creatorGustavo Batista de Menezespt_BR
dc.creatorFrank Kjeldsenpt_BR
dc.creatorThiago Verano-Bragapt_BR
dc.date.accessioned2023-10-30T21:06:07Z-
dc.date.available2023-10-30T21:06:07Z-
dc.date.issued2022-01-06-
dc.citation.volume11pt_BR
dc.citation.spage788482pt_BR
dc.citation.epage18pt_BR
dc.identifier.doihttps://doi.org/10.3389/fcimb.2021.788482pt_BR
dc.identifier.issn2235-2988pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/60284-
dc.description.resumoTrypanosoma cruzi invades non-professional phagocytic cells by subverting their membrane repair process, which is dependent on membrane injury and cell signaling, intracellular calcium increase, and lysosome recruitment. Cells lacking lysosome-associated membrane proteins 1 and 2 (LAMP1 and LAMP2) are less permissive to parasite invasion but more prone to parasite intracellular multiplication. Several passages through a different intracellular environment can significantly change T. cruzi’s gene expression profile. Here, we evaluated whether one single passage through LAMP-deficient (KO) or wild-type (WT) fibroblasts, thus different intracellular environments, could influence T. cruzi Y strain trypomastigotes’ ability to invade L6 myoblasts and WT fibroblasts host cells. Parasites released from LAMP2 KO cells (TcY-L2−/−) showed higher invasion, calcium signaling, and membrane injury rates, for the assays in L6 myoblasts, when compared to those released from WT (TcY-WT) or LAMP1/2 KO cells (TcY-L1/2−/−). On the other hand, TcY-L1/2−/− showed higher invasion, calcium signaling, and cell membrane injury rates, for the assays in WT fibroblasts, compared to TcY-WT and TcY-L1/2−/−. Albeit TcY-WT presented an intermediary invasion and calcium signaling rates, compared to the others, in WT fibroblasts, they induced lower levels of injury, which reinforces that signals mediated by surface membrane protein interactions also have a significant contribution to trigger host cell calcium signals. These results clearly show that parasites released from WT or LAMP KO cells are distinct from each other. Additionally, these parasites’ ability to invade the cell may be distinct depending on which cell type they interact with. Since these alterations most likely would reflect differences among parasite surface molecules, we also evaluated their proteome. We identified few protein complexes, membrane, and secreted proteins regulated in our dataset. Among those are some members of MASP, mucins, trans-sialidases, and gp63 proteins family, which are known to play an important role during parasite infection and could correlate to TcY-WT, TcY-L1/2−/−, and TcY-L2−/− biological behavior.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICASpt_BR
dc.publisher.departmentFARMACIA - FACULDADE DE FARMACIApt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIApt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE FISIOLOGIA E BIOFÍSICApt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIApt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE PARASITOLOGIApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofFrontiers in Celular and Infection Microbiologypt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectTrypanosoma cruzipt_BR
dc.subjectChagas diseasept_BR
dc.subjectLAMP proteinspt_BR
dc.subjectParasite invasionpt_BR
dc.subjectIntracellular environmentpt_BR
dc.subjectProteomic analysispt_BR
dc.subjectTMT (tandem mass tags)pt_BR
dc.subject.otherTrypanosoma cruzipt_BR
dc.subject.otherDoença de Chagaspt_BR
dc.subject.otherProteínaspt_BR
dc.subject.otherParasitospt_BR
dc.titleBiological and Molecular Effects of Trypanosoma cruzi Residence in a LAMP-Deficient Intracellular Environmentpt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.frontiersin.org/articles/10.3389/fcimb.2021.788482/fullpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-2823-4530pt_BR
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