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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/61148
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dc.creatorAlice de Araújo Ferreira Silvapt_BR
dc.creatorCarolina Nemésio de Barros Pereirapt_BR
dc.creatorDanilo Rocha Diaspt_BR
dc.creatorFrederico Santos Lagespt_BR
dc.creatorKatia Lucy Melo Maltospt_BR
dc.creatorAllyson Nogueira Moreirapt_BR
dc.creatorElton Gonçalves Zenobiopt_BR
dc.creatorIvana Márcia Alves Dinizpt_BR
dc.date.accessioned2023-11-20T20:10:47Z-
dc.date.available2023-11-20T20:10:47Z-
dc.date.issued2022-
dc.citation.volume36pt_BR
dc.citation.spagee090pt_BR
dc.citation.epage11pt_BR
dc.identifier.doihttps://doi.org/10.1590/1807-3107bor-2022.vol36.0090pt_BR
dc.identifier.issn1807-3107pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/61148-
dc.description.resumoThe topical glucocorticoid budesonide has been prescribed before and after sinus lift surgery as adjuvant drug treatment for maxillary sinus membrane inflammation. However, there is no study on the effects of budesonide on the regenerative process of bone grafting biomaterials. We investigated the effect of the association of budesonide with some biomaterials on the growth and differentiation capacity of pre-osteoblastic cells (MC3T3-E1 subclone 4). Xenogeneic (Bio-Oss and Bio-Gen) and synthetic hydroxyapatites (Osteogen, Bonesynth, and HAP-91) were tested in conditioned medium (1% w/v). The conditioned medium was then supplemented with budesonide (0.5% v/v). Cell viability was assessed using the MTT assay (48, 96, and 144 h), and mineralized nodules were quantified after 14 days of culture using the Alizarin Red Staining. Alkaline phosphatase activity was assessed through the release of thymolphthalein at day seven. All biomaterials showed little or no cytotoxicity. The Bio-Gen allowed significantly less growth than the control group regardless of the experimental time. Regarding differentiation potential of MC3T3-E1, the HAP-91-conditioned medium showed remarkable osteoinductive properties. In osteodifferentiation, the addition of budesonide favored the formation of mineral nodules when cells were cultured in medium conditioned with synthetic materials, whereas it weakened the mineralization potential of cells cultured in xenogeneic medium. Regardless of whether budesonide was added or not, Osteogen and Bio-Oss showed higher alkaline phosphatase activity than the other groups. Budesonide may improve bone formation when associated with synthetic biomaterials. Conversely, the presence of this glucocorticoid weakens the mineralization potential of pre-osteoblastic cells cultured with xenogeneic hydroxyapatites.pt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofBrazilian Oral Researchpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectEnxertospt_BR
dc.subjectBiomateriaispt_BR
dc.subjectBone Substitutespt_BR
dc.subjectHydroxyapatitespt_BR
dc.subjectGlucocorticoidspt_BR
dc.subjectBudesonidept_BR
dc.subject.otherOdontologiapt_BR
dc.subject.otherTransplantespt_BR
dc.subject.otherSubstitutos Ósseospt_BR
dc.titleGrafting biomaterials associated to topical glucocorticoid: effects on pre-osteoblastic cells (MC3T3-E1)pt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.scielo.br/j/bor/a/fMBvtLFt3BPszdRXhFpGXjh/?lang=enpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-6229-4778pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-7574-5697pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-9194-8774pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-7013-7043pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-7022-5593pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0537-1535pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-4261-0037pt_BR
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