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Use este identificador para citar o ir al link de este elemento: http://hdl.handle.net/1843/61840
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Campo DCValorIdioma
dc.creatorAmanda Nadia Dinizpt_BR
dc.creatorFrancisco Carlos Faria Lobatopt_BR
dc.creatorRodrigo Otávio Silveira Silvapt_BR
dc.creatorLoren Nery Fontoura Mourapt_BR
dc.creatorDiogo Soares Gonçalves Cruzpt_BR
dc.creatorCarlos Augusto de Oliveira Juniorpt_BR
dc.creatorHenrique César Pereira Figueiredopt_BR
dc.creatorJoão Luís Reis Cunhapt_BR
dc.creatorEduardo Garcia Vilelapt_BR
dc.creatorEdward J. Kuijperpt_BR
dc.creatorMark H Wilcoxpt_BR
dc.date.accessioned2023-12-07T21:25:09Z-
dc.date.available2023-12-07T21:25:09Z-
dc.date.issued2022-08-26-
dc.citation.volume17pt_BR
dc.citation.issue8pt_BR
dc.citation.spagee0273013pt_BR
dc.citation.epage21pt_BR
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0273013pt_BR
dc.identifier.issn1932-6203pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/61840-
dc.description.resumoClostridioides (Clostridium) difficile infection (CDI) is an evolving global healthcare problem, and owing to the diverse and dynamic molecular epidemiology of C. difficile, new strains continue to emerge. In Brazil, only two cases of CDI due to the so called hypervirulent PCR ribotype (RT) 027 belonging to clade 2 have ever been reported, whereas incidence of CDI due to another “hypervirulent” RT078 (clade 5) has not yet been reported. In contrast, novel clade 2 strains have been identified in different hospitals. To better understand the epidemiology of CDIs in Brazil, this study aimed to genotypically and phenotypically characterize three novel Brazilian clade 2 strains (RT883, 884, and 885) isolated from patients with confirmed CDI. In addition, to better understand the circulating RTs, a two-year sampling was conducted in patients from the same hospital and in several domestic and wild animal species. The three strains examined showed lower production of A/B toxins than the control RT027, although two of these strains harbored a truncated tcdC gene. All strains showed swimming motility similar to that of RT027, while RT883 showed higher spore production than the reference strain. In the in vivo hamster model, the lethality of all strains was found to be similar to that of RT027. Both cgMLST and cgMLSA analyses revealed a high genetic similarity among the three-novel clade 2 isolates. In the two-year survey in animals and humans, RT883, 884, and 885 were not detected; however, three new RTs (RT988, RT989, and RT990) were isolated, two of which were genetically related to the three previously reported clade 2 strains. RT106 and RT126 were most frequently detected in humans (47.9%) and animals (57.9%), respectively. Furthermore, RT027 and RT078 were not detected in humans. The results of this study suggest that these novel clade 2 strains have virulence potential and that new strains from clade 2 continue to emerge in our setting, indicating the need for long-term local surveillance.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentMED - DEPARTAMENTO DE CLÍNICA MÉDICApt_BR
dc.publisher.departmentMEDICINA - FACULDADE DE MEDICINApt_BR
dc.publisher.departmentVET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofPLoS ONEpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectProgramas de Rastreamentopt_BR
dc.subjectVirulênciapt_BR
dc.subjectBrasilpt_BR
dc.subject.otherProgramas de Rastreamentopt_BR
dc.subject.otherVirulênciapt_BR
dc.subject.otherBrasilpt_BR
dc.titleCharacterization of the virulence of three novel clade 2 Clostridioides (Clostridium) difficile strains and a two-year screening in animals and humans in Brazilpt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0273013pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-5287-5779pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-8577-6849pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4565-2868pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-0074-2488pt_BR
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