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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/62264
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dc.creatorIvana Domljanovicpt_BR
dc.creatorMaria Taskovapt_BR
dc.creatorPâmella Miranda de Mourapt_BR
dc.creatorGerald Weberpt_BR
dc.creatorKira Astakhovapt_BR
dc.date.accessioned2024-01-02T12:47:19Z-
dc.date.available2024-01-02T12:47:19Z-
dc.date.issued2020-
dc.citation.volume3pt_BR
dc.citation.issue1pt_BR
dc.citation.spage1pt_BR
dc.citation.epage13pt_BR
dc.identifier.doihttps://doi.org/10.1038/s42004-020-00362-5pt_BR
dc.identifier.issn2399-3669pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/62264-
dc.description.resumoDetection of nucleic acids is crucial to the study of their basic properties and consequently to applying this knowledge to the determination of pathologies such as cancer. In this work, our goal is to determine new trends for creating diagnostic tools for cancer driver mutations. Herein, we study a library of natural and modified oligonucleotide duplexes by a combination of optical and theoretical methods. We report a profound effect of additives on the duplexes, including nucleic acids as an active crowder. Unpredictably and inconsistent with DNA+LNA/RNA duplexes, locked nucleic acids contribute poorly to mismatch discrimination in the DNA+LNA/DNA duplexes. We develop a theoretical framework that explains poor mismatch discrimination in KRAS oncogene. We implement our findings in a bead-bait genotyping assay to detect mutated human cancer RNA. The performance of rationally designed probes in this assay is superior to the LNA-primer polymerase chain reaction, and it agrees with sequencing data.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICX - DEPARTAMENTO DE FÍSICApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofcommunications chemistry-
dc.rightsAcesso Abertopt_BR
dc.subjectOptical and theoretical studypt_BR
dc.subjectNucleic acid probespt_BR
dc.subject.otherBiofísicapt_BR
dc.subject.otherÁcidos nucleicospt_BR
dc.subject.otherÓticapt_BR
dc.titleOptical and theoretical study of strand recognition by nucleic acid probespt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.nature.com/articles/s42004-020-00362-5pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-9727-2496pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8876-1864pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-2935-1571pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-4878-0301pt_BR
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