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http://hdl.handle.net/1843/62481| Type: | Artigo de Periódico |
| Title: | Anti-serum validation for use in immunohistochemistry for trypanosoma cruzi detection |
| Authors: | Paulo Hernane Rabeloazevedo Marcelo Antônio Pascoal Xavier Glenda Nicioli da Silva Priscilla Almeida da Costa Mol Cláudia Martins Carneiro Geraldo Brasileiro Filho |
| Abstract: | The detection of Trypanosoma cruzi in tissue samples is important in many situations, such as testing of the reactivation of the infection. The detection of T. cruzi nests in endomyocardial biopsies (EMB) may be useful to evaluate graft rejection. Given their scarcity, such nests are not routinely identified. To increase the diagnosis sensitivity, immunohistochemistry (IHC) may serve as a promising strategy. Here, we validate an antiserum for the detection of T. cruzi infection by IHC. Methods: We used 1) positive controls (PCs) – 13 EMB, 12 skin biopsies, and 1 heart with T. cruzi nests as sections stained with hematoxylin and eosin (HE); 2) negative controls – a) 10 explant hearts and 10 EMB with no amastigote nests or clinical/laboratory signs of chagasic infection; and b) eight samples with leishmaniasis, toxoplasmosis, or histoplasmosis; and 3) Cases – 31 EMB of chagasic patients with no parasite nests in HE sections but detected positive for T. cruzi DNA by polymerase chain reaction. As a primary antibody, a hyperimmune serum from T. cruzi-infected rabbits was used. Results: IHC results were positive for 21 of 26 PCs (80.8%) and one case of cutaneous leishmaniasis. In 4 of 31 cases, IHC revealed nests (12.9%), which were undetected by conventional histological examination. Conclusions: This study shows that IHC with the tested antiserum increases the sensitivity of the diagnosis and may be recommended for routine use in EMB analyses of cardiac transplant patients with Chagas disease. |
| Subject: | Chagas Disease Heart Transplantation Immunohistochemistry Trypanosoma cruzi |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | MED - DEPARTAMENTO DE ANATOMIA PATOLÓGICA E MEDICINA LEGAL |
| Rights: | Acesso Aberto |
| metadata.dc.identifier.doi: | 10.1590/0037-8682-0011-2018 |
| URI: | http://hdl.handle.net/1843/62481 |
| Issue Date: | 2018 |
| metadata.dc.url.externa: | https://doi.org/10.1590/0037-8682-0011-2018 |
| metadata.dc.relation.ispartof: | Revista da Sociedade Brasileira de Medicina Tropical |
| Appears in Collections: | Artigo de Periódico |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Anti-serum validation for use in immunohistochemistry pdfa.pdf | 8.63 MB | Adobe PDF | View/Open |
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