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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/64867
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dc.creatorLilian Areal Marquespt_BR
dc.creatorSimone Cristine Semprebonpt_BR
dc.creatorAndressa Megumi Niwapt_BR
dc.creatorGláucia Fernanda Rocha D'epiropt_BR
dc.creatorDaniele Sartoript_BR
dc.creatorÂngelo de Fátimapt_BR
dc.creatorLúcia Regina Ribeiropt_BR
dc.creatorMário Sérgio Mantovanipt_BR
dc.date.accessioned2024-02-28T14:43:02Z-
dc.date.available2024-02-28T14:43:02Z-
dc.date.issued2016-
dc.citation.volume389pt_BR
dc.citation.spage1279pt_BR
dc.citation.epage1288pt_BR
dc.identifier.doihttps://doi.org/10.1007/s00210-016-1292-9pt_BR
dc.identifier.issn0028-1298pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/64867-
dc.description.resumoMonastrol is an allosteric inhibitor of the mitotic kinesin Eg5 that exhibits an antiproliferative effect against several cell lines. We investigated the antiproliferative effect of monastrol on human breast adenocarcinoma cells (MCF-7) and mammary epithelial cells (HB4a, non-tumoral). Monastrol treatment decreased cell viability only in MCF-7 tumor cells. Real-time cell growth kinetic analysis showed a decrease in the proliferation of MCF-7 cells exposed to monastrol, while in the HB4a cells, only a concentration of 100 μM was able to induce this effect. In a cell cycle analysis, exposure of MCF-7 cells to monastrol led to an increased population of cells in both the G1 and G2/M phases. In HB4a cells, the proportion of cells in the G2/M phase was increased. Monastrol led to an increased mitotic index in both cell lines. Monastrol was not able to induce cell death by apoptosis in any of the cell lines studied. Gene expression analysis was performed to measure the mRNA levels of cell cycle genes, DNA damage indicator gene, and apoptotic related genes. Treatment with monastrol induced in MCF-7 cells a 5-fold increase in the mRNA levels of the CDKN1A gene, an inhibitor of CDKs related with cell cycle arrest in response a stress stimulus, and a 2-fold decrease in CDKN1C mRNA levels in HB4a cells. These results provide evidence that monastrol has a greater antiproliferative effect on MCF-7 tumor cells compared with non-tumor HB4a cells; however, no selective is observed.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorshipOutra Agênciapt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICX - DEPARTAMENTO DE QUÍMICApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofNaunyn-Schmiedeberg's Archives of Pharmacologypt_BR
dc.rightsAcesso Restritopt_BR
dc.subjectMonastrolpt_BR
dc.subjectKinesin 5pt_BR
dc.subjectBreast non-Tumor Cellpt_BR
dc.subjectMCF-7pt_BR
dc.subjectHB4apt_BR
dc.subject.otherQuímicapt_BR
dc.subject.otherFarmacologiapt_BR
dc.subject.otherAgentes antineoplásicospt_BR
dc.subject.otherProteínas celularespt_BR
dc.subject.otherAdenocarcinomapt_BR
dc.subject.otherCitotoxidade de mediação celularpt_BR
dc.titleAntiproliferative activity of monastrol in human adenocarcinoma (MCF-7) and non-tumor (HB4a) breast cellspt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://link.springer.com/article/10.1007/s00210-016-1292-9#article-infopt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-9857-8681pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-7550-8509pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-9294-222Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-1151-2613pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0465-9932pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-2344-5590pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-5268-6508pt_BR
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