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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/65285
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dc.creatorJuliana Barbosa Salibapt_BR
dc.creatorRodrigo Lambert Oréficept_BR
dc.creatorMeriem Tekayapt_BR
dc.creatorLaura Kowalczukpt_BR
dc.creatorMin Zhaopt_BR
dc.creatorFrancine Behar-Cohenpt_BR
dc.creatorLorena Carla Vieirapt_BR
dc.creatorGabriella Maria Fernandes Cunhapt_BR
dc.creatorGisele Rodrigues da Silvapt_BR
dc.creatorSílvia Ligório Fialhopt_BR
dc.creatorArmando da Silva Cunha Júniorpt_BR
dc.creatorElodie Bousquetpt_BR
dc.creatorMarie-Christine Naudpt_BR
dc.creatorEliane Ayrespt_BR
dc.date.accessioned2024-03-05T17:55:14Z-
dc.date.available2024-03-05T17:55:14Z-
dc.date.issued2016-04-
dc.citation.volume57pt_BR
dc.citation.issue4pt_BR
dc.citation.spage1671pt_BR
dc.citation.epage1679pt_BR
dc.identifier.doi10.1167/iovs.15-18127pt_BR
dc.identifier.issn0146-0404pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/65285-
dc.description.resumoPURPOSE. Targeted drug delivery to the ocular tissues remains a challenge. Biodegradable intraocular implants allow prolonged controlled release of drugs directly into the eye. In this study, we evaluated an anterior suprachoroidal polyurethane implant containing dexamethasone polyurethane dispersions (DX-PUD) as a drug delivery system in the rat model of endotoxin-induced uveitis (EIU). METHODS. In vitro drug release was studied using PUD implants containing 8%, 20%, and 30% (wt/wt) DX. Cytotoxicity of the degradation products of DX-PUD was assessed on human ARPE19 cells using 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) test. Shortterm ocular biocompatibility of suprachoroidal DX-PUD implants was evaluated in normal rat eyes. Endotoxin-induced uveitis was then induced in rat eyes preimplanted with DX-PUD. Clinical examination was performed at 24 hours; eyes were used to assess inflammatory cell infiltration and macrophage/microglial activation. Cytokine and chemokine expression in the iris/ciliary body and in the retina was investigated using quantitative PCR. Feasibility of anterior suprachoroidal PUD implantation was also tested using postmortem human eyes. RESULTS. A burst release was followed by a sustained controlled release of DX from PUD implants. By-products of the DX-PUD were not toxic to human ARPE-19 cells or to rat ocular tissues. Dexamethasone-PUD implants prevented EIU in rat eyes, reducing inflammatory cell infiltration and inhibiting macrophage/microglial activation. Dexamethasone-PUD downregulated proinflammatory cytokines/chemokines (IL-1b, IL-6, cytokine-induced neutrophil chemoattractant [CINC]) and inducible nitric oxide synthase (iNOS) and upregulated IL-10 anti-inflammatory cytokine. Polyurethane dispersion was successfully implanted into postmortem human eyes. CONCLUSIONS. Dexamethasone-PUD implanted in the anterior suprachoroidal space may be of interest in the treatment of intraocular inflammation.pt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentENG - DEPARTAMENTO DE ENGENHARIA METALÚRGICApt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOSpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofInvestigative Ophthalmology & Visual Science-
dc.rightsAcesso Abertopt_BR
dc.subjectPolyurethanept_BR
dc.subjectImplantpt_BR
dc.subjectSuprachoroidal spacept_BR
dc.subjectDrug deliverypt_BR
dc.subjectEndotoxin-induced uveitispt_BR
dc.subject.otherImplantes de medicamentopt_BR
dc.subject.otherUveítept_BR
dc.subject.otherOftalmopatiaspt_BR
dc.subject.otherUveíte induzido quimicamentept_BR
dc.titleAnti-inflammatory effect of dexamethasone controlled released from anterior suprachoroidal polyurethane implants on endotoxin-induced uveitis in ratspt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://iovs.arvojournals.org/article.aspx?articleid=2513119&resultClick=1pt_BR
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