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http://hdl.handle.net/1843/65285
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Campo DC | Valor | Idioma |
---|---|---|
dc.creator | Juliana Barbosa Saliba | pt_BR |
dc.creator | Rodrigo Lambert Oréfice | pt_BR |
dc.creator | Meriem Tekaya | pt_BR |
dc.creator | Laura Kowalczuk | pt_BR |
dc.creator | Min Zhao | pt_BR |
dc.creator | Francine Behar-Cohen | pt_BR |
dc.creator | Lorena Carla Vieira | pt_BR |
dc.creator | Gabriella Maria Fernandes Cunha | pt_BR |
dc.creator | Gisele Rodrigues da Silva | pt_BR |
dc.creator | Sílvia Ligório Fialho | pt_BR |
dc.creator | Armando da Silva Cunha Júnior | pt_BR |
dc.creator | Elodie Bousquet | pt_BR |
dc.creator | Marie-Christine Naud | pt_BR |
dc.creator | Eliane Ayres | pt_BR |
dc.date.accessioned | 2024-03-05T17:55:14Z | - |
dc.date.available | 2024-03-05T17:55:14Z | - |
dc.date.issued | 2016-04 | - |
dc.citation.volume | 57 | pt_BR |
dc.citation.issue | 4 | pt_BR |
dc.citation.spage | 1671 | pt_BR |
dc.citation.epage | 1679 | pt_BR |
dc.identifier.doi | 10.1167/iovs.15-18127 | pt_BR |
dc.identifier.issn | 0146-0404 | pt_BR |
dc.identifier.uri | http://hdl.handle.net/1843/65285 | - |
dc.description.resumo | PURPOSE. Targeted drug delivery to the ocular tissues remains a challenge. Biodegradable intraocular implants allow prolonged controlled release of drugs directly into the eye. In this study, we evaluated an anterior suprachoroidal polyurethane implant containing dexamethasone polyurethane dispersions (DX-PUD) as a drug delivery system in the rat model of endotoxin-induced uveitis (EIU). METHODS. In vitro drug release was studied using PUD implants containing 8%, 20%, and 30% (wt/wt) DX. Cytotoxicity of the degradation products of DX-PUD was assessed on human ARPE19 cells using 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) test. Shortterm ocular biocompatibility of suprachoroidal DX-PUD implants was evaluated in normal rat eyes. Endotoxin-induced uveitis was then induced in rat eyes preimplanted with DX-PUD. Clinical examination was performed at 24 hours; eyes were used to assess inflammatory cell infiltration and macrophage/microglial activation. Cytokine and chemokine expression in the iris/ciliary body and in the retina was investigated using quantitative PCR. Feasibility of anterior suprachoroidal PUD implantation was also tested using postmortem human eyes. RESULTS. A burst release was followed by a sustained controlled release of DX from PUD implants. By-products of the DX-PUD were not toxic to human ARPE-19 cells or to rat ocular tissues. Dexamethasone-PUD implants prevented EIU in rat eyes, reducing inflammatory cell infiltration and inhibiting macrophage/microglial activation. Dexamethasone-PUD downregulated proinflammatory cytokines/chemokines (IL-1b, IL-6, cytokine-induced neutrophil chemoattractant [CINC]) and inducible nitric oxide synthase (iNOS) and upregulated IL-10 anti-inflammatory cytokine. Polyurethane dispersion was successfully implanted into postmortem human eyes. CONCLUSIONS. Dexamethasone-PUD implanted in the anterior suprachoroidal space may be of interest in the treatment of intraocular inflammation. | pt_BR |
dc.format.mimetype | pt_BR | |
dc.language | eng | pt_BR |
dc.publisher | Universidade Federal de Minas Gerais | pt_BR |
dc.publisher.country | Brasil | pt_BR |
dc.publisher.department | ENG - DEPARTAMENTO DE ENGENHARIA METALÚRGICA | pt_BR |
dc.publisher.department | FAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS | pt_BR |
dc.publisher.initials | UFMG | pt_BR |
dc.relation.ispartof | Investigative Ophthalmology & Visual Science | - |
dc.rights | Acesso Aberto | pt_BR |
dc.subject | Polyurethane | pt_BR |
dc.subject | Implant | pt_BR |
dc.subject | Suprachoroidal space | pt_BR |
dc.subject | Drug delivery | pt_BR |
dc.subject | Endotoxin-induced uveitis | pt_BR |
dc.subject.other | Implantes de medicamento | pt_BR |
dc.subject.other | Uveíte | pt_BR |
dc.subject.other | Oftalmopatias | pt_BR |
dc.subject.other | Uveíte induzido quimicamente | pt_BR |
dc.title | Anti-inflammatory effect of dexamethasone controlled released from anterior suprachoroidal polyurethane implants on endotoxin-induced uveitis in rats | pt_BR |
dc.type | Artigo de Periódico | pt_BR |
dc.url.externa | https://iovs.arvojournals.org/article.aspx?articleid=2513119&resultClick=1 | pt_BR |
Aparece en las colecciones: | Artigo de Periódico |
archivos asociados a este elemento:
archivo | Descripción | Tamaño | Formato | |
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Anti-Inflammatory Effect of Dexamethasone Controlled.pdf | 956.09 kB | Adobe PDF | Visualizar/Abrir |
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