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Use este identificador para citar o ir al link de este elemento: http://hdl.handle.net/1843/79420
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Campo DCValorIdioma
dc.creatorTales Fernandoda Silvapt_BR
dc.creatorRafael de Assis Glóriapt_BR
dc.creatorThiago Jesus de Sousapt_BR
dc.creatorMonique Ferrary Americopt_BR
dc.creatorAndria dos Santos Freitaspt_BR
dc.creatorMarcus Vinicius Canário Vianapt_BR
dc.creatorLuís Cláudio Lima de Jesuspt_BR
dc.creatorLigia Carolina da Silva Pradopt_BR
dc.creatorNathalie Daniept_BR
dc.creatorOlivia Ménardpt_BR
dc.creatorMarie‑Françoise Cochept_BR
dc.creatorDidier Dupontpt_BR
dc.creatorJulien Jardinpt_BR
dc.creatorAmanda Dias Borgespt_BR
dc.creatorSimone Odília Antunes Fernandespt_BR
dc.creatorValbert Nascimento Cardosopt_BR
dc.creatorBertram Brenigpt_BR
dc.creatorEnio Ferreirapt_BR
dc.creatorRodrigo Profetapt_BR
dc.creatorFlavia Figueira Aburjailept_BR
dc.creatorRodrigo Dias Oliveira de Carvalhopt_BR
dc.creatorPhilippe Langellapt_BR
dc.creatorYves Le Loirpt_BR
dc.creatorClaire Cherbuypt_BR
dc.creatorGwénaël Janpt_BR
dc.creatorVasco Azevedopt_BR
dc.creatorÉric Guédonpt_BR
dc.date.accessioned2025-01-22T21:58:05Z-
dc.date.available2025-01-22T21:58:05Z-
dc.date.issued2023-11-27-
dc.citation.volume23pt_BR
dc.citation.spage1pt_BR
dc.citation.epage30pt_BR
dc.identifier.doihttps://doi.org/10.1186/s12866-023-03112-4pt_BR
dc.identifier.issn1471-2180pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/79420-
dc.description.resumoBackground: Probiotics have gained attention for their potential maintaining gut and immune homeostasis. They have been found to confer protection against pathogen colonization, possess immunomodulatory effects, enhance gut barrier functionality, and mitigate inflammation. However, a thorough understanding of the unique mechanisms of effects triggered by individual strains is necessary to optimize their therapeutic efficacy. Probiogenomics, involving high-throughput techniques, can help identify uncharacterized strains and aid in the rational selection of new probiotics. This study evaluates the potential of the Escherichia coli CEC15 strain as a probiotic through in silico, in vitro, and in vivo analyses, comparing it to the well-known probiotic reference E. coli Nissle 1917. Genomic analysis was conducted to identify traits with potential beneficial activity and to assess the safety of each strain (genomic islands, bacteriocin production, antibiotic resistance, production of proteins involved in host homeostasis, and proteins with adhesive properties). In vitro studies assessed survival in gastrointestinal simulated conditions and adhesion to cultured human intestinal cells. Safety was evaluated in BALB/c mice, monitoring the impact of E. coli consumption on clinical signs, intestinal architecture, intestinal permeability, and fecal microbiota. Additionally, the protective effects of both strains were assessed in a murine model of 5-FU-induced mucositis. Results: CEC15 mitigates inflammation, reinforces intestinal barrier, and modulates intestinal microbiota. In silico analysis revealed fewer pathogenicity-related traits in CEC15, when compared to Nissle 1917, with fewer toxin-associated genes and no gene suggesting the production of colibactin (a genotoxic agent). Most predicted antibiotic-resistance genes were neither associated with actual resistance, nor with transposable elements. The genome of CEC15 strain encodes proteins related to stress tolerance and to adhesion, in line with its better survival during digestion and higher adhesion to intestinal cells, when compared to Nissle 1917. Moreover, CEC15 exhibited beneficial effects on mice and their intestinal microbiota, both in healthy animals and against 5FU-induced intestinal mucositis.pt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICASpt_BR
dc.publisher.departmentVET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofMicrobiologia BMCpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectProbioticspt_BR
dc.subjectProbiogenomicspt_BR
dc.subjectEscherichia coli CEC15pt_BR
dc.subjectGenomicspt_BR
dc.subjectEscherichia coli Nissle 1917pt_BR
dc.subjectMucositispt_BR
dc.subjectImmunomodulationpt_BR
dc.subjectGastrointestinal tractpt_BR
dc.subject.otherProbióticospt_BR
dc.subject.otherPatologiapt_BR
dc.subject.otherSistema gastrointestinalpt_BR
dc.subject.otherSistema imunológicopt_BR
dc.titleComprehensive probiogenomics analysis of the commensal Escherichia coli CEC15 as a potential probiotic strainpt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-023-03112-4#Abs1pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0237-9398pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4607-6364pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-6501-9533pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-2498-7933pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-7017-6437pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-7708-3033pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4974-8269pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-5304-6561pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-0520-9391pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-9332-4888pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-9428-4494pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-6139-5187pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-7597-9602pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-7635-9656pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-1835-0303pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0487-3058pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-1067-1882pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-5960-4341pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0355-1065pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-3088-4199pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8694-3381pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4775-2280pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0901-4447pt_BR
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