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Use este identificador para citar o ir al link de este elemento: http://hdl.handle.net/1843/79569
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Campo DCValorIdioma
dc.creatorMaria Sissa Pereira Sant'anapt_BR
dc.creatorSara Ferreira dos Santos Costapt_BR
dc.creatorMaísa Pereira da Silvapt_BR
dc.creatorRoberta Rayra Martins Chavespt_BR
dc.creatorThaís dos Santos Fontes Pereirapt_BR
dc.creatorEduardo Morato de Oliveirapt_BR
dc.creatorRicardo Martínez Pedrazapt_BR
dc.creatorWagner Henriques de Castropt_BR
dc.creatorCarolina Cavaliéri Gomespt_BR
dc.creatorRicardo Santiago Gomezpt_BR
dc.creatorFelipe Paiva Fonsecapt_BR
dc.date.accessioned2025-01-31T14:01:21Z-
dc.date.available2025-01-31T14:01:21Z-
dc.date.issued2021-05-
dc.citation.volume50pt_BR
dc.citation.issue5pt_BR
dc.citation.spage478pt_BR
dc.citation.epage484pt_BR
dc.identifier.doihttps://doi.org/10.1111/jop.13155pt_BR
dc.identifier.issn1600-0714pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/79569-
dc.description.resumoBackground: BRAF p.V600E is reported in up to 80% of ameloblastomas. Despite the high frequency, the presence of this mutation in different histopathological areas of the tumour has not been investigated. This information has an important role in the use of BRAF p.V600E assessment as an auxiliary tool in the differential diagnosis between unicystic ameloblastoma and other odontogenic cystic lesions, especially when only incisional biopsies are available. Therefore, the purpose of the present study was to investigate BRAF p.V600E heterogeneity in unicystic ameloblastoma. Methods: Five cases of ameloblastoma and two dentigerous cysts were analysed. The regions exhibiting different microscopic characteristics were selected from each ameloblastoma case and manually dissected. TaqMan allele-specific qPCR or Sanger sequencing was performed to determine BRAF p.V600E status. Results: We screened the mutation in a small cohort of UA and no molecular heterogeneity was found. Four cases of ameloblastoma (80%) exhibited BRAF p.V600E in all different areas evaluated. One case did not harbour the mutation in any microscopic region analysed. The BRAF mutation was absent in the dentigerous cysts. Conclusion: Ameloblastomas appear to exhibit a homogeneous profile regarding the BRAF p.V600E no matter what histological feature is observed under light microscopy, suggesting that this molecular test may contribute to establish the correct diagnosis in cases microscopically resembling other odontogenic lesions.pt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAO - FACULDADE DE ODONTOLOGIApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of Oral Pathology & Medicinept_BR
dc.rightsAcesso Restritopt_BR
dc.subjectBRAFpt_BR
dc.subjectBRAF p.V600Ept_BR
dc.subjectAmeloblastomapt_BR
dc.subjectDentigerous cystpt_BR
dc.subjectOdontogenic tumourpt_BR
dc.subject.otherAmeloblastomapt_BR
dc.subject.otherProto-oncogene proteins B-rafpt_BR
dc.subject.otherDentigerous cystpt_BR
dc.subject.otherOdontogenic tumorspt_BR
dc.titleBRAF p.V600E status in epithelial areas of ameloblastoma with different histological aspects: implications to the clinical practicept_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/jop.13155pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-0199-1884pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-8770-8009pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-6657-4547pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-5150-9227pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-2483-8695pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-6182-9232pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-0046-0026pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-2745-2878pt_BR
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