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Use este identificador para citar o ir al link de este elemento: http://hdl.handle.net/1843/80109
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Campo DCValorIdioma
dc.creatorAdriana de Souza Andradept_BR
dc.creatorSofia Oliveira Campospt_BR
dc.creatorJamile Diaspt_BR
dc.creatorMarco Antônio Campospt_BR
dc.creatorErna Geessien Kroonpt_BR
dc.date.accessioned2025-02-14T22:17:24Z-
dc.date.available2025-02-14T22:17:24Z-
dc.date.issued2023-09-03-
dc.citation.volume20pt_BR
dc.citation.spage1pt_BR
dc.citation.epage13pt_BR
dc.identifier.doihttps://doi.org/10.1186/s12985-023-02168-ypt_BR
dc.identifier.issn1743-422Xpt_BR
dc.identifier.urihttp://hdl.handle.net/1843/80109-
dc.description.resumoBackground: Dengue virus (DENV) is a Flaviviridae member classified into four antigenically distinct serotypes (DENV 1, 2, 3, and 4) and further subdivided genotypes. DENV3 is subdivided into four or five genotypes, depending on the classification adopted. Despite their high genetic proximity, as revealed by phylogenetic complete polyprotein analysis, DENV3 MG-20 and DENV3 PV_BR showed different neurovirulence in mice models. Our group identified six amino acid mutations in protein E, including the E62K and E123Q, which may affect interactions of hydrophobic clusters on domain II, thus leading to the observed differences in the studied viruses. Methods Human glioblastoma cells (U251) derived from a malignant glioblastoma tumor by explant technique were infected by the DENV3 GIL1 isolates DENV3 MG-20 and DENV3 PV_BR and analyzed by plaque assays and titration, optical, immunofluorescence, and transmission electronic microscopy. Results: The two isolates showed different cytopathic effects (CPE) and fusogenic patterns, further confirmed by indirect immunofluorescence. Transmission electron microscopy revealed intense cytopathic effects in DENV3 MG-20 infected U251 cells, displaying endoplasmic reticulum hypertrophy and turgid vesicles with proteins and multiple viruses, distinct from DENV3 PV_BR infected cells. It is hypothesized that the different amino acids in the DENV3 MG-20 isolate are related to an increased membrane fusion ability in viral infection, thus facilitating immune system evasion and increased chances of central nervous system cell infection. Conclusion: These results emphasize the biological differences between the isolates, which could be a critical factor in host-virus interaction and severe dengue development. Our study presents comparative results of highly similar isolates with the potential to generate more subsidies for a deeper understanding of the DENV pathogenesis. The neurotropism of the isolate DENV3 MG-20 (belonging to the DENV3 GI L1 genotype) showing infection of nervous system cells (U251) could contribute to understanding neurological dengue disease.pt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofVirology Journalpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectDengue viruspt_BR
dc.subjectSyncytiumpt_BR
dc.subjectFlaviviridaept_BR
dc.subjectHuman glioblastoma cellspt_BR
dc.subjectCytopathic effectpt_BR
dc.subject.otherVirologiapt_BR
dc.subject.otherVírus da Denguept_BR
dc.subject.otherArbovíruspt_BR
dc.titleDengue virus 3 genotype I (GI) lineage 1 (L1) isolates elicit differential cytopathic effect with syncytium formation in human glioblastoma cells (U251)pt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://virologyj.biomedcentral.com/articles/10.1186/s12985-023-02168-y#rightslinkpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-2394-8219pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-9915-260Xpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-2721-3826pt_BR
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