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Use este identificador para citar o ir al link de este elemento: http://hdl.handle.net/1843/81096
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Campo DCValorIdioma
dc.creatorBeatriz Senra Álvares da Silva Santospt_BR
dc.creatorJoão Luís Reis-Cunhapt_BR
dc.creatorIsadora Cota Carvalhopt_BR
dc.creatorJulia Machado Caetano Costapt_BR
dc.creatorBarbara Longopt_BR
dc.creatorGrazielle Cossenzo Florentino Galinaript_BR
dc.creatorP. H.S.M. Dinizpt_BR
dc.creatorGabriel Moreira de Mello Mendespt_BR
dc.creatorFlávio Guimarães da Fonsecapt_BR
dc.creatorJônatas Santos Santos Abrahãopt_BR
dc.creatorHerman Sander Mansurpt_BR
dc.creatorMaria Fátima Leitept_BR
dc.date.accessioned2025-03-28T23:41:02Z-
dc.date.available2025-03-28T23:41:02Z-
dc.date.issued2022-11-04-
dc.citation.volume12pt_BR
dc.citation.issue49pt_BR
dc.identifier.doi10.1039/D2RA05096Jpt_BR
dc.identifier.issn2046-2069pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/81096-
dc.description.resumoThe current COVID-19 pandemic presents one of the greatest challenges in human history. There is a consensus that the rapid and accurate diagnosis of COVID-19 directly affects procedures to avoid dissemination, promote treatments, and favor the prognosis of infected patients. This interdisciplinary study aims at designing new synthetic peptides inspired by the SARS-CoV-2 spike protein (SARS-CoV-2S) to produce rapid detection tests relying on nanomaterial-based colorimetric properties. Hence, in silico analyses of SARS-CoV-2S were performed using advanced bioinformatic simulation tools and algorithms. Five novel peptide sequences were proposed, and three were selected (P2, J4, and J5) based on their prospective reactivity against positive serum from naturally COVID 19-infected humans. Next, hyperimmune sera against the selected peptides were produced in rabbits. Concurrently, gold nanoparticles (AuNP) were synthesized using a green aqueous method under mild conditions through in situ reduction by trisodium citrate salt. They were extensively characterized by their morphological, physicochemical, and optical properties. The AuNPs demonstrated colloidal chemical stability in aqueous media, with an average size of approximately 29 nm (metallic core), and zeta potential before and after bioconjugation of −43 mV and −31 mV, respectively. Moreover, they presented an intense reddish-bluish color due to the surface plasmon resonance (SPR) effect, with maxima at λ = 525 nm and 536 nm, before and after bioconjugation, respectively, evidencing their applicability as colorimetric biomarkers for antigen–antibody immunoassay detection. To develop a rapid COVID-19 diagnosis test using lateral flow assay (LFA), semi-purified anti-SARS-CoV-2S sera against the three selected peptides were bioconjugated to the AuNPs as the highly optically sensitive agents using a considerably low antibody concentration (0.2 mg mL−1). All tested peptide sequences (P2, J4, and J5) induced antibodies capable of identifying the presence of SARS-CoV-2 virus inactivate suspensions (1 : 10, 1 : 100, or 1 : 1000 dilutions). For LFA positive test control, an anti-rabbit antibody was used. In summary, this research comprises several contributions and advances to the broad and multidisciplinary field of nanomaterials-based immunodiagnosis tools, encompassing: (a) the novelty of designing and synthesizing new immunogenic peptides inspired by SARS-CoV-2 virus epitopes using in silico bioinformatics; (b) the peptides induced the immune response in rabbit animal model producing hyperimmune serum; (c) the semi-purified hyperimmune serum rendered effective antibodies to detect SARS-CoV-2 virus in cell suspension; (d) colloidal gold nanoparticles were produced and bioconjugated to the antibodies for qualitative colorimetric detection. As the overall result of this study, it was designed, developed, produced, and validated a new simple, rapid, and sensitive LFA diagnostic test for the SARS-CoV-2 virus using a nanotechnology-based qualitative colorimetric assay, which can be envisioned as promising nanoplatforms for detecting other diseases.pt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentVET - DEPARTAMENTO DE MEDICINA VETERINÁRIA PREVENTIVApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofRSC advancespt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectSARS-CoV-2pt_BR
dc.subjectCOVID-19pt_BR
dc.subjectPandemiapt_BR
dc.subjectNanotecnologiapt_BR
dc.subjectImunologiapt_BR
dc.subject.otherCOVID-19 (Doença)pt_BR
dc.subject.otherSaúde Públicapt_BR
dc.subject.otherNanotecnologiapt_BR
dc.subject.otherImunologiapt_BR
dc.titleNanotechnology meets immunology towards a rapid diagnosis solution: the covid-19 outbreak challengept_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://pubs.rsc.org/en/content/articlelanding/2022/ra/d2ra05096jpt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-9399-3691pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-1497-4397pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0003-1427-8401pt_BR
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