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Use este identificador para citar o ir al link de este elemento: http://hdl.handle.net/1843/82928
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Campo DCValorIdioma
dc.creatorPatrícia Carlos Caldeirapt_BR
dc.creatorÉrica Leandro Marciano Vieirapt_BR
dc.creator. Alexandre de Andrade Sousapt_BR
dc.creatorAntonio Lucio Teixeira Juniorpt_BR
dc.creatorMaria Cássia Ferreira de Aguiarpt_BR
dc.date.accessioned2025-06-12T20:37:53Z-
dc.date.available2025-06-12T20:37:53Z-
dc.date.issued2017-10-
dc.citation.volume46pt_BR
dc.citation.issue9pt_BR
dc.citation.spage703pt_BR
dc.citation.epage709pt_BR
dc.identifier.doihttps://doi.org/10.1111/jop.12575pt_BR
dc.identifier.issn1600-0714pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/82928-
dc.description.resumoBackground: The aim was to explore the immunophenotype of neutrophils and lymphocytes and the inflammatory mediators in patients with oral squamous cell carcinoma, comparing with controls; and to associate with clinicopathological data. Methods: Blood was collected from 13 patients and 13 controls. The immunophenotype of neutrophils (CD66b, CD16, CD11a, arginase-1), T lymphocytes (CD4, CD8) and the intracellular cytokine production (IL-10, TNF, IFN-γ) was evaluated by flow cytometry. Plasma concentration of sVCAM-1, sTNF-RI, sTNF-RII, and IL-1β was measured by ELISA. MPO, Lipocalin-2/NGAL, sICAM-1, and p-selectin were quantified by Luminex assay. The excised tumors were submitted to immunohistochemistry for neutrophils (CD66b) and lymphocytes (CD3, CD4, CD8). Association with clinical data was explored. P values <.05 were considered significant. Results: Patients presented higher percentage of neutrophils and lower lymphocytes, resulting a higher neutrophil/lymphocyte ratio than controls. They also presented higher percentage of neutrophils expressing CD66b+ , CD66b+ Arginase-1+ , CD66b+ IL10+ , CD66b+ TNF+ , CD66b+ Arginase-1+ IL-10+ , and lower CD66b+ CD16+ CD11a+ and CD66b+ Arginase-1+ TNF+ . CD66b+ neutrophils were detected in all tumors, with a CD66b+ /CD3+ ratio of 0.40. Patients showed higher concentration of plasmatic sVCAM-1 and lower Lipocalin-2/NGAL. Patients with good outcome presented lower percentage of neutrophils, higher percentage of lymphocytes, and lower NLR than patients who died. Conclusion: The amount and immunophenotype of neutrophils and lymphocytes differ between patients and healthy individuals, with a pro-tumorigenic profile of neutrophils. As these cells also get within tumor microenvironment, they possibly exert systemic and local functions in cancer pathogenesis. The association of neutrophil count with outcome corroborates recent studies and this merits further investigation for applicability as a prognosticator.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICApt_BR
dc.publisher.departmentMED - DEPARTAMENTO DE CIRURGIApt_BR
dc.publisher.departmentMED - DEPARTAMENTO DE CLÍNICA MÉDICApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of Oral Pathology & Medicinept_BR
dc.rightsAcesso Restritopt_BR
dc.subjectELISApt_BR
dc.subjectFlow cytometrypt_BR
dc.subjectImmunohistochemistrypt_BR
dc.subjectNeutrophilspt_BR
dc.subjectOutcomept_BR
dc.subjectSquamous cell carcinomapt_BR
dc.subject.otherImmunohistochemistrypt_BR
dc.subject.otherEnzyme-linked immunosorbent assaypt_BR
dc.subject.otherFlow cytometrypt_BR
dc.subject.otherNeutrophilspt_BR
dc.subject.otherCarcinoma, squamous cellpt_BR
dc.subject.otherProteinspt_BR
dc.subject.otherTumor necrosis factorspt_BR
dc.subject.otherImmunophenotypingpt_BR
dc.subject.otherT-lymphocytespt_BR
dc.subject.otherCytokinespt_BR
dc.titleImmunophenotype of neutrophils in oral squamous cell carcinoma patientspt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/jop.12575pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-9179-0145pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4147-5614pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-4204-9334pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0002-9621-5422pt_BR
dc.identifier.orcidhttps://orcid.org/0000-0001-5134-3466pt_BR
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