Modulação da atividade funcional de fagócitos mononucleares de sangue periférico por citocinas na presença de Entamoeba histolytica

Abstract

Entamoeba histolytica (E. histolytica) is the agent that causes the amoebiasis, which is a disease with significant morbidity and mortality. Phagocytic cells and cytokines appear to be important in amoebiasis, but the influence of these cells and cytokines in protozoan infections is not totally elucidated. The aim of this study was to analyze the supernatant of cultures of MN cells with E. histolytica to determine: 1) the levels of the cytokines IFN-, TGF, IL-4 and IL-17; 2) the functional activity of MN cells after incubation with cytokines. Blood samples were collected from 60 volunteer donors healthy. The levels of IFN- , TGF - , IL-4 and IL-17 were quantified in cell culture supernatants -trophozoite. The superoxide release, leukophagocytosis, amoebicided activity, apoptosis and intracellular calcium release were analyzed during interactions of MN phagocytes and amoebae. It was observed that the MN cells, regardless of kind of cytokines, in the presence of E. histolytica increased superoxide release. The MN cells treated with cytokines reduced leukophagocytosis is capacity of trophozoites. The amebicided activity and apoptosis rates were higher when the MN phagocytes were treated by cytokines. The highest amebicidal and apoptosis rates were observed when the MN cells were treated by TGF-. The cytokines TGF- and IL-17 increased the release of intracellular calcium by MN phagocytes. The results suggest that cytokines play a beneficial role to the host by activation of MN cells for E. histolytica. They also suggest that during leukophagocytosis is occur death of amoebae the modulating the functional activity of phagocytes MN IFN-, TGF-, IL-4 and IL-17 appear to be essential in controlling the amoebic infections.