Efeitos indiretos da tolerância oral melhoram o processo de reparo de lesões excisionais na pele de camundongos

Abstract

The parenteral injection of ovalbunima (OVA) in OVA-orally tolerant mice inhibits inflammation and immune responses to others antigens injected concomitantly. This inhibitory effect named indirect effect of oral tolerance also inhibits inflammation after incisional skin lesion. Herein, we investigate if the i.p. injection of an orally tolerated protein inhibit the inflammatory process in excisional lesions in the skin of adult mice and alter the repair process of these lesions. C57BL/6 mice were made orally tolerant to OVA by drinking a solution of egg white for 3 days (Tolerant) and control mice drank tap water (Immune). Seven days after oral treatment both groups were immunized i.p. with OVA+Al(OH)3 immediately before the skin lesions under anesthesia using a dermatological punch with 6.5 mm diameter. Another control group (Lesion) received the lesion without immunization. Mice were sacrificed 1, 3, 5, 7, 14 and 40 days after and the skin around the lesion was fixed in formalin and embedded in paraffin for histological analysis (HE staining, Toluidine Blue and Gomori Trichrome) or fixed in 80% methanol and 20% DMSO and embedded in paraplast for immunofluorescency analysis with anti-Ly-6C / G (Gr-1), anti-CD3, anti-alpha-SMA, anti-tenascin-C, anti-anti-collagen I and collagen III. In addition, photographs of the wounds were obtained from day 0 to day 14 to measure their areas. Tolerant and Immune groups showed greater reduction in wound area on days 3 and 5 after lesions. Tolerant mice had fewer inflammatory cells and fibroblasts in the wound area and a reduced scar; in tolerant mice collagen I and collagen III in the wound area were more similar to the fibers of the intact skin and tenascin-C was increased. We conclude that the indirect effects of oral tolerance reduce inflammation and scar tissue after skin wound healing