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http://hdl.handle.net/1843/ODON-AXNQVC| Type: | Tese de Doutorado |
| Title: | Participação de citocinas relacionadas às respostas Th17/Treg na inflamação periapical inflamatória crônica |
| Authors: | Andre Oliveira Naufel de Toledo |
| First Advisor: | Maria Cassia Ferreira de Aguiar |
| First Co-advisor: | Mila Fernandes Moreira Madeira |
| First Referee: | Gil Moreira Júnior |
| Second Referee: | Giovanna Ribeiro Souto |
| Third Referee: | Soraia Macari |
| metadata.dc.contributor.referee4: | Ricardo Reis Oliveira |
| Abstract: | A doenca periapical inflamatoria e uma sequela da infeccao e necrose pulpar. Ela representa a resposta de defesa do hospedeiro a agressao proveniente do canal radicular. Assim, lesoes periapicais cronicas se desenvolvem como resposta a manutencao da inflamacao e a reabsorcao ossea. As celulas T reguladoras (Treg) e celulas T helper (Th) 17 (Th17) tem papel fundamental na regulacao da resposta imunologica. Forkhead box P3 (FoxP3) e o fator de transcricao para as celulas Treg, mas a diferenciacao e maturacao das celulas T naive em Treg ou Th17 depende tambem de citocinas especificas como o fatortransformador de crescimento (TGF-), interleucina 10 (IL-10), interleucina 17 (IL-17), interleucina 6 (IL-6) e interleucina 21 (IL-21). Outras citocinas participam na modulacao da atividade de Treg e Th17 como as quimiocinas CCL4 e CCL20 com funcao de recrutamento destas celulas para atuacao no processo inflamatorio. O papel de Treg e Th17 tem sido muito estudado em doencas autoimunes, mas ainda pouco avaliado na patogenia das lesoes inflamatorias periapicais cronicas. Para este estudo foram utilizadas oitenta e sete amostrasde tecido periapical humano para realizacao de analises morfologicas edosagem de citocinas por meio de ensaio imunoenzimatico (ELISA) (TGF-, IL-10, IL-17, CCL4, CCL20). As amostras de tecido periapical foram coletadas de tres grupos: grupo controle (dentes higidos), grupo de dentes com necrose pulpar e lesao periapical e grupo de dentes com necrose pulpar sem lesao periapical. Observamos alta expressao de CCL4 e TGF- no grupo com lesao periapical quando comparado com os grupos sem lesao e uma correlacao positiva entre CCL20 e IL-17, alem de um aumento na expressao de CCL20 no grupo com lesao periapical quando comparado ao controle. Nossas observacoesimplicam que essas duas caracteristicas, tanto pro-inflamatorias quantoimunossupressoras, estao presentes na lesao periapical cronica, ocorrendo de maneira simultanea e com caracteristicas de co-estimulacao, como resultado do intenso trabalho da resposta imunologica do hospedeiro contra o processo inflamatorio proveniente das bacterias intracanais e seus subprodutos. |
| Abstract: | Inflammatory periapical disease is a sequel of the infection and pulp necrosis. It represents the host defense response to aggression from the root canal. Chronic periapical lesions are developed in response to the maintenance of inflammation and bone resorption. Regulatory T cells (Tregs) and Th17 cells play a key role in regulating the immune response with opposite functions. Forkhead box P3 (FoxP3) is the master transcription factor for Treg cells, but differentiation and maturation of naive T cells in Treg or Th17 also depend on specific cytokines such as transforming growth factor (TGF), interleukin 10 (IL -10), interleukin-17 (IL-17), interleukin-6 (IL-6) and interleukin-21 (IL-21). Other cytokinesparticipate in the modulation of the Treg and Th17 activity, as the chemokines CCL4 and CCL20 with function of recruitment of these cells to act in the inflammatory process. The role of Treg and Th17 has been better studied in autoimmune diseases, but sparsely evaluated in the pathogenesis of chronic periapical inflammatory lesions. Eighty-six human samples were used to perform histologic analysis and the cytokine dosage by enzyme-linked immunosorbent assay (ELISA) (TGF-, IL-10, IL-17, CCL4, CCL20) in the periapical tissue of three groups: control group, group of teeth with pulp necrosis and periapicallesion and group of teeth with necrosis without periapical lesion. We observed high expression of CCL4 and TGF- in the group with periapical lesion in comparison with the groups without lesion and demonstrated increased expression of CCL20 in the group with periapical lesion when compared to control. Our findings imply that these two characteristics, pro-inflammatory and immunosuppressive, are present in the chronic periapical lesion, occurring simultaneously and with co-stimulation characteristics, as a result of the intense actionof the host's immune response against the inflammatory process coming from intracanal bacteria and their by-products. |
| Subject: | Células Th17 Doenças periapicais Periodontite periapical Quimiocinas Citocinas Odontologia |
| language: | Português |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| Rights: | Acesso Aberto |
| URI: | http://hdl.handle.net/1843/ODON-AXNQVC |
| Issue Date: | 21-Jul-2017 |
| Appears in Collections: | Teses de Doutorado |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| tese_vesao_5.pdf | 2.41 MB | Adobe PDF | View/Open |
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