Estresse do transporte em rã – touro: respostas hematológicas e bioquímicas

Abstract

The study aimed to evaluate the recovery of bullfrog (Lithobates catesbeianus) homeostasis after transport. The experiment was carried out in a completely randomized design in a factorial scheme with an additional treatment [5 x 2 + (1)]. Five times after transport were evaluated (0 - immediately after transport, 6, 12, 24 and 48 hours), in both genders and a control treatment (condition before transport). The treatments consisted of five repetitions, the animal being the experimental unit. Seventy frogs, 35 males (352.12 ± 34.67 g) and 35 females (375.87 ± 48.10 g) were fasted for 42 hours before transport. Of these, 10 (five males and five females) were used to collect blood before transportation (control). The other 60 were transported for nine hours in five PVC pipes of 1 m and 100 mm in diameter, in the density of 12 animals / pipe. At the end of the transport, the frogs were distributed in five flooded bays (1.15 x 1.06 m) in the density of 12 frogs / bay (six males and six females), until the pre-established evaluation times. For each evaluation time, blood was collected from 10 frogs (five males and five females). With blood samples, the following analyzes were performed: blood glucose, lactate, total proteins, albumin, globulins, albumin / globulin (A:G) ratio, triglycerides, total cholesterol, AST, ALT, hemoglobin, hematocrit, total erythrocyte count and absolute hematimetric indices (VCM, HCM and CHCM). There was no significant interaction between the collection times and the gender of the frogs. Glycemia and lactate increased in frogs from time 0 h, but reestablished at other times. The total protein increased in frogs from time 0 h, but decreased in time 24 and 48 h. Albumin increased in frogs from time 0 and 6 h, but decreased in the collection of 48 h. Globulins increased in frogs from time 0 h, but decreased in time 24 and 48 h. Triglycerides increased in frogs from time 0 h, but decreased in other hours. Total cholesterol decreased progressively in frogs from time 0 h and was higher in male. AST increased in frogs from time 0 and 6 h, but decreased from time 12 h. ALT increased in frogs from time 0 h, but decreased in time 24 and 48 h. The hematocrit reduced in the time frogs 48 h. The number of erythrocytes increased in frogs from time 0 h, but decreased in time 48 h. Hemoglobin decreased in frogs over 48 h. VCM and HCM decreased in animals from time 0 h. CHCM increased in the frogs evaluated at 24 h. The variables respond differently to stress. However, between 6 and 12 hours, most of the variables evaluated have already returned to the same condition as the control treatment. The reduction of some variables after 24 h may be a consequence of the energy deficit caused by prolonged fasting.