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Type:	Artigo de Periódico
Title:	pH-sensitive, long-circulating liposomes as an alternative tool to deliver doxorubicin into tumors: a feasibility animal study
Authors:	Juliana de Oliveira Silva Renata Salgado Fernandes Sávia Caldeira de Araújo Lopes Valbert Nascimento Cardoso Elaine Amaral Leite Geovanni Dantas Cassali Maria Cristina Marzola Domenico Rubello Mônica Cristina de Oliveira André Luís Branco de Barros
Abstract:	Purpose: Therapeutic agents used in chemotherapy have low specificity leading to undesired severe side effects. Hence, the development of drug delivery systems that improve drug specificity, such as liposome moieties, is an alternative to overcome chemotherapy limitations and increase antitumor efficacy. In this study, the biodistribution profile evaluation of pH-sensitive long-circulating liposomes (SpHL) containing [99mTc]DOX in 4T1 tumor-bearing BALB/c mice is described. Procedures: [99mTc]DOX was radiolabeled by direct method. Liposomes were prepared and characterized. [99mTc]DOX was encapsulated into liposomes by freezing and thawing. Circulation time for SpHL-[99mTc]DOX was determined by measuring the blood activity from healthy animals. Biodistribution studies were carried out in tumor-bearing mice at 1, 4, and 24 h after injection. Results: Blood levels of the SpHL-[99mTc]DOX declined in a biphasic manner, with an α half-life of 14.1 min and β half-life of 129.0 min. High uptake was achieved in the liver and spleen, due to the macrophages captured. Moreover, tumor uptake was higher than control tissue, resulting in high tumor-to-muscle ratios, indicating higher specificity for the tumor area. Conclusion: [99mTc]DOX was successfully encapsulated in liposomes. Biodistribution indicated high tumor-to-muscle ratios in breast tumor-bearing BALB/c mice. In summary, these results showed the higher accumulation of SpHL-[99mTc]DOX in the tumor area, suggesting selective delivery of doxorubicin into tumor.
Subject:	Lipossomos Doxorrubicina Neoplasias Câncer Tecnécio
language:	eng
metadata.dc.publisher.country:	Brasil
Publisher:	Universidade Federal de Minas Gerais
Publisher Initials:	UFMG
metadata.dc.publisher.department:	FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS FAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS ICB - DEPARTAMENTO DE PATOLOGIA
Rights:	Acesso Restrito
metadata.dc.identifier.doi:	https://doi.org/10.1007/s11307-016-0964-7
URI:	http://hdl.handle.net/1843/40099
Issue Date:	Dec-2016
metadata.dc.url.externa:	https://link.springer.com/article/10.1007/s11307-016-0964-7
metadata.dc.relation.ispartof:	Molecular Imaging and Biology
Appears in Collections:	Artigo de Periódico

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