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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/40515
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dc.creatorCarolina Sheng Whei Miawpt_BR
dc.creatorGláucia Celeste de Souza Amânciopt_BR
dc.creatorViviane Netto Rochapt_BR
dc.creatorJovita Eugênia Gazzinelli Cruz Madeirapt_BR
dc.creatorScheilla Vitorino Carvalho de Souzapt_BR
dc.date.accessioned2022-03-28T20:34:11Z-
dc.date.available2022-03-28T20:34:11Z-
dc.date.issued2017-
dc.citation.volume9pt_BR
dc.citation.issue1pt_BR
dc.citation.spage105pt_BR
dc.citation.epage114pt_BR
dc.identifier.doi10.3920/QAS2015.0739pt_BR
dc.identifier.issn17578361pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/40515-
dc.description.resumoConsidering expansion of genetically modified organisms and the basic principle of consumers’ right to access information about products, legislations of several countries establish a limit for labelling transgenic food. Qualitative tests based on the polymerase chain reaction (PCR) have been recommended. However, validation of qualitative methods is still a critical point in the quality management of food analysis laboratories. A screening method for the detection of Roundup Ready (RR) soy in soybeans by nested PCR was validated by a novel qualitative approach, in a single-laboratory, considering all fundamental parameters for an adequate evaluation of fitness for purpose. Blank samples of soybeans and formulations containing 0.001 to 1% of RR soy were analysed. Agarose gel electrophoresis and fluorimetry techniques were compared in terms of the quantification of extracted DNA. False-positive rate obtained was 0%, with selectivity and reliability rates of 100.0% for both techniques. Sensitivity and reliability rates varied between 23.3 and 100.0% (agarose gel) and between 30.0 and 100.0% (fluorimetry), respectively. Levels above 0.030% presented 100.0% positive results. Unreliable regions were estimated by non-linear models, and the calculated detection limits were 0.0067 and 0.0047%, for agarose gel electrophoresis and fluorimetry, respectively. Accordance and concordance values of 1.0 were obtained for levels near the regulated limit. The method was considered fit for screening purposes. Analysis of commercial samples demonstrated the applicability of the method and the compliance with Brazilian legislation.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE ALIMENTOSpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofQuality Assurance and Safety of Crops & Foodspt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectGenetically modified organismspt_BR
dc.subjectRoundup Ready soypt_BR
dc.subjectScreening methodpt_BR
dc.subjectLabelling regulationspt_BR
dc.subjectSingle-laboratory validationpt_BR
dc.subjectRotulagempt_BR
dc.subject.otherTecnologia de alimentospt_BR
dc.subject.otherSojapt_BR
dc.titleSingle-laboratory validation of a method for detection of Roundup Ready soy in soybeans: application of new strategies for qualitative validationpt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.qascf.com/index.php/qas/article/view/185pt_BR
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