Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/40673
Type: Artigo de Periódico
Title: Occurrence and enumeration of rope-producing spore forming bacteria in flour and their spoilage potential in different bread formulations
Authors: Ana Paula Maciel Pereira
Graziele Cristina Stradiotto
Luísa Freire
Verônica Ortiz Alvarenga
Aline Crucello
Leticia Lauandos Pozza Morassi
Fabiana P. Silva
Anderson de Souza Sant'Ana
Abstract: This study aimed to investigate the prevalence of spore forming rope-producing microorganisms in different types of flour and to evaluate the potential for spoilage of the isolates through the simulation of the bread manufacturing process. Mesophilic and thermophilic aerobic spore forming bacteria were enumerated in 100 flour samples. Strains isolates were evaluated for their ability to produce amylase and cause rope spoilage in different bread formulations. Amylase-producing strains were identified through 16S rRNA sequencing. The wholemeal flour from industry presented the highest aerobic mesophilic spore forming bacteria counts (3.1 log spores/g). A total of 327 strains were isolated from flours, among which 45 produced amylase. These isolates were identified as Bacillus licheniformis (62%), B. sonorensis (20%) B. cereus (11%), B. pumilus (2%), and Paenibacillus polymyxa (2%). All 45 strains were able to cause spoilage in bread with and without preservatives. The addition of calcium propionate and reduction of water activity and pH were able to prevent the development of rope for 7 days. The development of new formulations can help to assure the microbiological quality and safety of baked products.
Subject: Bactérias
Farinha
Pão
language: eng
metadata.dc.publisher.country: Brasil
Publisher: Universidade Federal de Minas Gerais
Publisher Initials: UFMG
metadata.dc.publisher.department: FAR - DEPARTAMENTO DE ALIMENTOS
Rights: Acesso Aberto
metadata.dc.identifier.doi: 10.1016/j.lwt.2020.110108
URI: http://hdl.handle.net/1843/40673
Issue Date: Nov-2020
metadata.dc.url.externa: https://www.sciencedirect.com/science/article/pii/S0023643820310975
metadata.dc.relation.ispartof: LWT - Food Science and Technology
Appears in Collections:Artigo de Periódico



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.