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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/41299
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dc.creatorIngrid Vasconcelospt_BR
dc.creatorPedro Henrique Reis da Silvapt_BR
dc.creatorDerick Rodrigues Davila Diaspt_BR
dc.creatorMaria Betânia de Freitas Marquespt_BR
dc.creatorWagner da Nova Musselpt_BR
dc.creatorTércio Assunção Pedrosapt_BR
dc.creatorMaria Elisa Scarpelli Ribeiro e Silvapt_BR
dc.creatorRoberto Fernando de Souza Freitaspt_BR
dc.creatorRicardo Geraldo de Sousapt_BR
dc.creatorChristian Fernandespt_BR
dc.date.accessioned2022-05-02T20:11:44Z-
dc.date.available2022-05-02T20:11:44Z-
dc.date.issued2020-11-
dc.citation.volume116pt_BR
dc.citation.spage1pt_BR
dc.citation.epage3pt_BR
dc.identifier.doi10.1016/j.msec.2020.111191pt_BR
dc.identifier.issn0928-4931pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/41299-
dc.description.resumoGliclazide is a sulfonylurea frequently prescribed for the management of type 2 diabetes mellitus in elderly patients and for patients with chronic renal or hepatic diseases. Even though it is considered a safer alternative, the drug can provoke side effects in some patients, especially hypoglycemia, due to the high interindividual variability. Therefore, the quantification of gliclazide in biological samples is usually recommended in order to assure efficacy and safety of the pharmacotherapy. However, due to the complexity of biological matrices, therapeutic monitoring can be very challenging, especially in the sample preparation step. For that reason, the synthesis and characterization of a novel and selective molecularly imprinted polymer (MIP) was proposed to be employed as sorbent for the extraction of gliclazide from human plasma samples by a molecularly imprinted solid-phase extraction (MISPE) procedure. Synthesis conditions were optimized (monomer, crosslinker and porogen) and the polymer was characterized for its morphological, physicochemical and stability properties. The influence of drug concentration, solvent composition and pH on the coefficient of distribution (Kd) and imprinting factor (IF) were studied, as well as repeatability between batches and selectivity. A bioanalytical method was developed applying the developed MIP as sorbent in solid phase extraction and liquid chromatography using a Poroshell 120 C18 (100 × 4.6 mm, 4 μm) column, acetonitrile and 10 mM potassium phosphate buffer pH 3.0 (50:50) at a flow-rate of 1.2 mL/min as mobile phase, temperature of 30 °C, injection volume of 40 μL and detection at 230 nm. The best reaction yield, extraction capacity, and selectivity was obtained using 2-hydroxyethyl methacrylate (2-HEMA), ethyleneglycol dimethacrylate (EGDMA) and acetonitrile. The optimized MIP showed coefficient of distribution (Kd) of 59.85 μg/g, imprinting factor (IF) of 1.60, and selectivity for gliclazide and other sulfonylureas compared to possible concurrent drugs. The developed method by MISPE-HPLC-UV showed to be appropriate to determine gliclazide in human plasma samples.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorshipOutra Agênciapt_BR
dc.format.mimetypepdfpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE ALIMENTOSpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOSpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofMaterials Science and Engineering: Cpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectDiabetes mellituspt_BR
dc.subjectSulphonylureaspt_BR
dc.subjectMolecularly imprinted solid-phase extractionpt_BR
dc.subjectSample preparationpt_BR
dc.subject.otherDiabetes mellituspt_BR
dc.subject.otherGliclazidapt_BR
dc.titleSynthesis and characterization of a molecularly imprinted polymer (MIP) for solid-phase extraction of the antidiabetic gliclazide from human plasmapt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.sciencedirect.com/science/article/pii/S0928493120316891pt_BR
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