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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/41526
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dc.creatorRenata França Cassimiro Belopt_BR
dc.creatorJúlia Pereira Figueiredopt_BR
dc.creatorCarolina Mariana Nunespt_BR
dc.creatorRafael Pissinattipt_BR
dc.creatorScheilla Vitorino Carvalho de Souzapt_BR
dc.creatorRoberto Gonçalves Junqueirapt_BR
dc.date.accessioned2022-05-10T21:34:13Z-
dc.date.available2022-05-10T21:34:13Z-
dc.date.issued2017-05-15-
dc.citation.volume1053pt_BR
dc.citation.spage87pt_BR
dc.citation.epage100pt_BR
dc.identifier.doi10.1016/j.jchromb.2017.03.017pt_BR
dc.identifier.issn1570-0232pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/41526-
dc.description.resumoAn accelerated solvent extraction (ASE) procedure for use with gas chromatography-mass spectrometry (GC–MS) was optimized for the determination of eight polycyclic aromatic hydrocarbons (PAHs) in cocoa beans. Plackett-Burman and rotatable central composite design (RCCD) indicated that three variables affected the recoveries of PAHs during the extraction and purification steps: agitation time in the second liquid–liquid partition, weight of silica gel in the column, and volume of hexane for PAH elution from the column. After obtaining the optimal conditions, a single laboratory method validation was performed. Linearity was demonstrated for benzo[a]pyrene in the concentration range from 0.5 to 8.0 mg kg−1 of sample, corresponding to 1.25–20.0 μg kg−1 of cocoa on a fat basis. For the other analytes, linearity was observed from 0.75 to 8.0 μg kg−1 of sample (1.88–20.0 μg kg−1 of cocoa on a fat basis). Significant matrix effects were found for chrysene and benzo[b]fluoranthene. The precision of the method was verified with relative standard deviations (RSDs) ranging from 2.57 to 14.13% and from 4.36 to 19.77% under repeatability and intermediate precision conditions, respectively. The average recoveries of the eight PAHs ranged from 74.99 to 109.73%. These parameters, limits and measurement uncertainties met the performance criteria established by European Union regulations, except for the theoretical limit of detection for chrysene. The method was applied to the analysis of samples of Brazilian cocoa beans, and only one sample was found to have a PAH content above the maximum limit defined by the European Union legislation. This optimized and validated method is intended to be used as part of the official Brazilian monitoring programs investigating contaminants and residues in food.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorshipOutra Agênciapt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE ALIMENTOSpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of Chromatography Bpt_BR
dc.rightsAcesso Restritopt_BR
dc.subjectAccelerated solvent extractionpt_BR
dc.subjectGas chromatographypt_BR
dc.subjectMass spectrometrypt_BR
dc.subjectPolycyclic aromatic hydrocarbonspt_BR
dc.subjectSingle laboratory method validationpt_BR
dc.subjectCocoa beanpt_BR
dc.subject.otherTecnologia de alimentospt_BR
dc.subject.otherCacaupt_BR
dc.subject.otherCromatografia gasosapt_BR
dc.subject.otherEspectrometria de massaspt_BR
dc.titleAccelerated solvent extraction method for the quantification of polycyclic aromatic hydrocarbons in cocoa beans by gas chromatography–mass spectrometrypt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.sciencedirect.com/science/article/pii/S1570023216310431?via%3Dihubpt_BR
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