Predicting adhesion and biofilm formation boundaries on stainless steel surfaces by five Salmonella enterica strains belonging to different serovars as a function of pH, temperature and NaCl concentration

Juliana de Oliveira Moraes; Ellen Abreu da Cruz; Ênio Gomes Flôr Souza; Tereza Cristina Moreira de Oliveira; Verônica Ortiz Alvarenga; Wilmer Edgard Luera Penã; Anderson de Souza Sant'Ana; Marciane Magnani

Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/41620

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DC Field	Value	Language
dc.creator	Juliana de Oliveira Moraes	pt_BR
dc.creator	Ellen Abreu da Cruz	pt_BR
dc.creator	Ênio Gomes Flôr Souza	pt_BR
dc.creator	Tereza Cristina Moreira de Oliveira	pt_BR
dc.creator	Verônica Ortiz Alvarenga	pt_BR
dc.creator	Wilmer Edgard Luera Penã	pt_BR
dc.creator	Anderson de Souza Sant'Ana	pt_BR
dc.creator	Marciane Magnani	pt_BR
dc.date.accessioned	2022-05-12T20:14:50Z	-
dc.date.available	2022-05-12T20:14:50Z	-
dc.date.issued	2018-09-20	-
dc.citation.volume	281	pt_BR
dc.citation.spage	90	pt_BR
dc.citation.epage	100	pt_BR
dc.identifier.doi	10.1016/j.ijfoodmicro.2018.05.011	pt_BR
dc.identifier.issn	0168-1605	pt_BR
dc.identifier.uri	http://hdl.handle.net/1843/41620	-
dc.description.resumo	This study aimed to assess the capability of 97 epidemic S. enterica strains belonging to 18 serovars to form biofilm. Five strains characterized as strong biofilm-producers, belonging to distinct serovars (S. Enteritidis 132, S. Infantis 176, S. Typhimurium 177, S. Heidelberg 281 and S. Corvallis 297) were assayed for adhesion/biofilm formation on stainless steel surfaces. The experiments were conducted in different combinations of NaCl (0, 2, 4, 5, 6, 8 and 10% w/v), pH (4, 5, 6 and 7) and temperatures (8 °C, 12 °C, 20 °C and 35 °C). Only adhesion was assumed to occur when S. enterica counts were ≥3 and <5 log CFU/cm2, whereas biofilm formation was defined as when the counts were ≥5 log CFU/cm2. The binary responses were used to develop models to predict the probability of adhesion/biofilm formation on stainless steel surfaces by five strains belonging to different S. enterica serovars. A total of 99% (96/97) of the tested S. enterica strains were characterized as biofilm-producers in the microtiter plate assays. The ability to form biofilm varied (P < 0.05) within and among the different serovars. Among the biofilm-producers, 21% (20/96), 45% (43/96), and 35% (34/96) were weak, moderate and strong biofilm-producers, respectively. The capability for adhesion/biofilm formation on stainless steel surfaces under the experimental conditions studied varied among the strains studied, and distinct secondary models were obtained to describe the behavior of the five S. enterica tested. All strains showed adhesion at pH 4 up to 4% of NaCl and at 20 °C and 35 °C. The probability of adhesion decreased when NaCl concentrations were >8% and at 8 °C, as well as in pH values ≤ 5 and NaCl concentrations > 6%, for all tested strains. At pH 7 and 6, biofilm formation for S. Enteritidis, S. Infantis, S. Typhimurium, S. Heidelberg was observed up to 6% of NaCl at 35 °C and 20 °C. The predicted boundaries for adhesion were pH values < 5 and NaCl ≥ 4% and at temperatures <20 °C. For biofilm formation, the predicted boundaries were pH values < 5 and NaCl concentrations ≥ 2% and at temperatures <20 °C for all strains. The secondary models obtained describe the variability in boundaries of adhesion and biofilm formation on stainless steel by five strains belonging to different S. enterica serovars. The boundary models can be used to predict adhesion and biofilm formation ability on stainless steel by S. enterica as affected by pH, NaCl and temperature.	pt_BR
dc.description.sponsorship	CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico	pt_BR
dc.description.sponsorship	CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior	pt_BR
dc.language	eng	pt_BR
dc.publisher	Universidade Federal de Minas Gerais	pt_BR
dc.publisher.country	Brasil	pt_BR
dc.publisher.department	FAR - DEPARTAMENTO DE ALIMENTOS	pt_BR
dc.publisher.initials	UFMG	pt_BR
dc.relation.ispartof	International Journal of Food Microbiology	pt_BR
dc.rights	Acesso Restrito	pt_BR
dc.subject	Growth no-growth models	pt_BR
dc.subject	Food hygiene	pt_BR
dc.subject	Sanitization	pt_BR
dc.subject	Attachment	pt_BR
dc.subject	Surface	pt_BR
dc.subject	Foodborne pathogens	pt_BR
dc.subject.other	Salmonella enterica	pt_BR
dc.subject.other	Biofilme	pt_BR
dc.title	Predicting adhesion and biofilm formation boundaries on stainless steel surfaces by five Salmonella enterica strains belonging to different serovars as a function of pH, temperature and NaCl concentration	pt_BR
dc.type	Artigo de Periódico	pt_BR
dc.url.externa	https://www.sciencedirect.com/science/article/pii/S0168160518302459#!	pt_BR
Appears in Collections:	Artigo de Periódico

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