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Type: Artigo de Periódico
Title: A simple and rapid LC–MS/MS method for the determination of amphenicols in Nile tilapia
Authors: Letícia Rocha Guidi
Patrícia Amaral Souza Tette
Maria Beatriz de Abreu Glória
Christian Fernandes
Abstract: A quantitative method for analysis of amphenicols (chloramphenicol – CAP, thiamphenicol – TAP and florfenicol – FF) in Nile tilapia using LC–MS/MS is described. A simple sample preparation procedure was optimized using a Plackett-Burman design. The method was validated in accordance with Decision 2002/657/EC. Repeatability and reproducibility were less than 10.7% and 16%, respectively, for all compounds. Recoveries varied from 79.8% to 92.0%. CCα was 0.019, 54.81 and 54.93 μ−1 for CAP, FF and TAP, respectively. CCβ was 0.068, 64.88 and 58.91 μ−1 for CAP, FF and TAP, respectively. Limits of quantification (LOQ) were 12.5 μ−1 for FF and TAP and 0.15 μ−1 for CAP. Nile tilapia fillets (n = 32) analyzed did not contain chloramphenicol. Thiamphenicol was detected in one sample (3.1%) and florfenicol was detected in every sample, all of them at concentrations below the maximum residue limit.
Subject: Tecnologia de alimentos
Tilápia do Nilo
Cromatografia líquida
language: eng Brasil
Publisher: Universidade Federal de Minas Gerais
Publisher Initials: UFMG
metadata.dc.publisher.department: FAR - DEPARTAMENTO DE ALIMENTOS
Rights: Acesso Restrito
metadata.dc.identifier.doi: 10.1016/j.foodchem.2018.04.087
Issue Date: 1-Oct-2018
metadata.dc.relation.ispartof: Food Chemistry
Appears in Collections:Artigo de Periódico

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