Biodistribution of free and encapsulated 99mTc-fluconazole in an infection model induced by Candida albicans

Danielle Nogueira de Assis; Raquel Silva Araújo; Leonardo Lima Fuscaldi; Simone Odília Antunes Fernandes; Vanessa Carla Furtado Mosqueira; Valbert Nascimento Cardoso

Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/41627

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DC Field	Value	Language
dc.creator	Danielle Nogueira de Assis	pt_BR
dc.creator	Raquel Silva Araújo	pt_BR
dc.creator	Leonardo Lima Fuscaldi	pt_BR
dc.creator	Simone Odília Antunes Fernandes	pt_BR
dc.creator	Vanessa Carla Furtado Mosqueira	pt_BR
dc.creator	Valbert Nascimento Cardoso	pt_BR
dc.date.accessioned	2022-05-12T21:27:02Z	-
dc.date.available	2022-05-12T21:27:02Z	-
dc.date.issued	2018-03	-
dc.citation.volume	99	pt_BR
dc.citation.spage	438	pt_BR
dc.citation.epage	444	pt_BR
dc.identifier.doi	10.1016/j.biopha.2018.01.021	pt_BR
dc.identifier.issn	0753-3322	pt_BR
dc.identifier.uri	http://hdl.handle.net/1843/41627	-
dc.description.resumo	Background Candida spp is an etiologic agent of fungal infections in hospitals and resistance to treatment with antifungals has been extensively reported. Thus, it is very important to develop formulations that increase effectiveness with low toxicity. In this sense, nanocarriers have been investigated, once they modify drug biodistribution profile. Thus, this study aimed to evaluate the biodistribution of free and encapsulated 99mTc-fluconazole into nanocapsules (NCs) in an experimental immunosuppressed murine model of Candida albicans infection. Methods Fluconazole was radiolabeled with technetium-99 metastable (99mTc) and encapsulated into conventional (99mTc-Fluconazole-PLA-POLOX) and surface-modified (99mTc-Fluconazole-PLA-PEG) NCs by the interfacial deposition of the preformed biodegradable polymer [poly (D,L-lactic acid) (PLA) and PLA–PEG (polyethyleneglycol)] followed by solvent evaporation. The size distribution and zeta potential of the NCs preparations were determined in a Zetasizer by photon correlation spectroscopy and laser Doppler anemometry, respectively. Free and encapsulated 99mTc-fluconazole were administered intravenously in immunosuppressed mice bearing a local infection induced by Candida Albicans inoculation in the right thigh muscle. At pre-established time intervals, tissues and organs of interest were removed and radioactivity was measured in an automatic gamma radiation counter. Results The NCs diameter was between 200 and 400 nm with negative zeta potential values. Free 99mTc-fluconazole was more rapidly eliminated by the renal system compared to the encapsulated drug in NCs, which remained longer in blood circulation. The uptake of conventional NCs by mononuclear phagocyte system organs was higher than the one demonstrated by the surface-modified NCs. Both NCs remained longer in the infectious focus when compared to free 99mTc-fluconazole, but the results did not show a significant difference between NC formulations. Conclusion These data indicate that these NCs might represent a therapeutic alternative for the treatment of candidiasis, once they remain more time in the infectious focus, allowing high retention of 99mTc-fluconazole at this site.	pt_BR
dc.description.sponsorship	CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico	pt_BR
dc.description.sponsorship	FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais	pt_BR
dc.description.sponsorship	CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior	pt_BR
dc.language	eng	pt_BR
dc.publisher	Universidade Federal de Minas Gerais	pt_BR
dc.publisher.country	Brasil	pt_BR
dc.publisher.department	FAR - DEPARTAMENTO DE ALIMENTOS	pt_BR
dc.publisher.department	FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS	pt_BR
dc.publisher.initials	UFMG	pt_BR
dc.relation.ispartof	Biomedicine & Pharmacotherapy	pt_BR
dc.rights	Acesso Restrito	pt_BR
dc.subject	Infectious focus	pt_BR
dc.subject	Biodistribution	pt_BR
dc.subject	Nanocapsules	pt_BR
dc.subject	Technetium-99 metastable	pt_BR
dc.subject	Fluconazole	pt_BR
dc.subject	Candida spp	pt_BR
dc.subject.other	Candida albicans	pt_BR
dc.subject.other	Biodistribuição	pt_BR
dc.subject.other	Tecnécio-99 metaestável	pt_BR
dc.title	Biodistribution of free and encapsulated 99mTc-fluconazole in an infection model induced by Candida albicans	pt_BR
dc.type	Artigo de Periódico	pt_BR
dc.url.externa	https://www.sciencedirect.com/science/article/pii/S0753332217351478?via%3Dihub	pt_BR
Appears in Collections:	Artigo de Periódico

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