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http://hdl.handle.net/1843/41810| Type: | Artigo de Periódico |
| Title: | Use of chitosan as pharmaceutical excipient in ocular drug delivery systems: sterilization and pharmacokinetics |
| Authors: | Juçara Ribeiro França Leonardo Lima Fuscaldi Tatiana Gomes Ribeiro Giselle Foureaux Aina Liz Alves Cesar Rachel Oliveira Castilho Sebastião Cronemberger Anderson José Ferreira Simone Odília Antunes Fernandes Valbert Nascimento Cardoso André Augusto Gomes Faraco |
| Abstract: | The use of chitosan as a pharmaceutical excipient in the ocular field is already established. Nevertheless, some aspects related to its ocular administration, such as sterilization and excipient's pharmacokinetics, remain unclear. So, in this study, we evaluated those two relevant aspects, related to chitosan administration in eye. We used chitosan-based ocular inserts (CI) as formulation model. CI were produced by solvent/casting method and sterilized by saturated steam. Sterilization was confirmed by direct inoculation of inserts in suitable microbiological growth media. Physicochemical characterization of inserts before and after sterilization was performed. Results suggested that, although steam sterilization changed the arrangement of the matrix, the heat and the humidity did not modify the structure of the main polymeric chain. Pharmacokinetics of CI radiolabeled with technetium-99m (99mTc) was assessed by scintigraphic images and ex vivo biodistribution study, after ocular administration in male Wistar rats. Scintigraphic and images analysis and ex vivo biodistribution study showed that the insert remained mainly in the eye until 6 hr after administration and its degradation products began to migrate to the abdominal cavity after 18 hr. Together, these data represent an important step forward the manufacturing and the clinical application of CI in the ophthalmic field. |
| Subject: | Quitosana Farmacocinética Administração ocular de drogas |
| language: | eng |
| metadata.dc.publisher.country: | Brasil |
| Publisher: | Universidade Federal de Minas Gerais |
| Publisher Initials: | UFMG |
| metadata.dc.publisher.department: | FAR - DEPARTAMENTO DE ALIMENTOS FAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS FAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS ICB - DEPARTAMENTO DE MORFOLOGIA MED - DEPARTAMENTO DE OFTALMOLOGIA E OTORRINOLARINGOLOGIA |
| Rights: | Acesso Restrito |
| metadata.dc.identifier.doi: | 10.1002/jbm.b.34560 |
| URI: | http://hdl.handle.net/1843/41810 |
| Issue Date: | Jul-2020 |
| metadata.dc.url.externa: | https://onlinelibrary.wiley.com/doi/10.1002/jbm.b.34560 |
| metadata.dc.relation.ispartof: | Journal of Biomedical Materials Research Part B: Applied Biomaterials |
| Appears in Collections: | Artigo de Periódico |
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