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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/41860
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dc.creatorIara Rinco Silvapt_BR
dc.creatorAlysson Vinícius Bragapt_BR
dc.creatorMaria Beatriz de Abreu Glóriapt_BR
dc.creatorRenes de Resende Machadopt_BR
dc.creatorIsabela Costa Césarpt_BR
dc.creatorRenata Barbosa de Oliveirapt_BR
dc.date.accessioned2022-05-20T19:01:58Z-
dc.date.available2022-05-20T19:01:58Z-
dc.date.issued2020-07-15-
dc.citation.volume1149pt_BR
dc.citation.spage1pt_BR
dc.citation.epage7pt_BR
dc.identifier.doi10.1016/j.jchromb.2020.122180pt_BR
dc.identifier.issn1570-0232pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/41860-
dc.description.resumoRN104, named 2-[2-(cyclohexylmethylene)hydrazinyl)]-4-phenylthiazole, is a thiazolyl hydrazone derivative with promising antifungal activity. Pharmacokinetic profile of the RN104 was evaluated in mice plasma using a developed and validated bioanalytical method by LC-MS/MS. Clotrimazole was used as internal standard. The analytes were extracted by a protein precipitation procedure and separated on a C18 end-capped column and mobile phase composed of acetonitrile - 0.1% formic acid (85:15, v/v), in isocratic mode. Electrospray ionization in positive ionization mode (ESI + ) and multiple reaction monitoring (MRM) were employed using the transitions m/z 286.1 → m/z 176.1 (quantifier) and m/z 286.1 → m/z 112.2 (qualifier) for RN104 and m/z 345.2 → m/z 277.1 (quantifier) and m/z 345.2 → m/z 165.2 (qualifier) for internal standard. The method was validated and proved to be linear, accurate, precise, and selective over the range 0.625 to 40.0 ng/mL. The pharmacokinetic model that best fit the data was the bicompartmental model. The maximum plasmatic concentration was reached 20 min after administration (per os and intraperitoneal) and the highest plasma concentration of RN104 was found after per os administration at a dosage of 50 mg/kg compared to i.p. administration at 10 mg/kg.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE ALIMENTOSpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOSpt_BR
dc.publisher.departmentICX - DEPARTAMENTO DE QUÍMICApt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of Chromatography Bpt_BR
dc.rightsAcesso Restritopt_BR
dc.subjectAntifungalpt_BR
dc.subjectLiquid chromatography–tandem mass spectrometrypt_BR
dc.subjectPharmacokineticpt_BR
dc.subjectRN104pt_BR
dc.subject.otherAntifúngicopt_BR
dc.subject.otherCromatografia líquidapt_BR
dc.subject.otherEspectrometria de massaspt_BR
dc.subject.otherFarmacocinéticapt_BR
dc.subject.otherCamundongospt_BR
dc.titlePreclinical pharmacokinetic study of a new thiazolyl hydrazone derivative with antifungal activity in mice plasma by LC-MS/MSpt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.sciencedirect.com/science/article/pii/S1570023220303809?via%3Dihubpt_BR
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