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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/41861
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dc.creatorWilliam Gustavo de Limapt_BR
dc.creatorJulio César Moreira de Britopt_BR
dc.creatorValbert Nascimento Cardosopt_BR
dc.creatorSimone Odília Antunes Fernandespt_BR
dc.date.accessioned2022-05-20T19:19:42Z-
dc.date.available2022-05-20T19:19:42Z-
dc.date.issued2021-01-01-
dc.citation.volume156pt_BR
dc.citation.spage1pt_BR
dc.citation.epage13pt_BR
dc.identifier.doi10.1016/j.ejps.2020.105592pt_BR
dc.identifier.issn0928-0987pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/41861-
dc.description.resumoSkin infections caused by methicillin-resistant Staphylococcus aureus (MRSA) require the development of new and effective topical antibiotics. In this context, melittin, the main component of apitoxin, has a potent antibacterial effect. However, little is known regarding the anti-inflammatory potential this peptide in infection models, or its ability to induce clinically important resistance. Here, we aimed to conduct an in-depth characterization of the antibacterial potential of melittin in vitro and evaluate the pharmaceutical potential of an ointment containing melittin for the treatment of non-surgical infections induced by MRSA. The minimum inhibitory concentration of melittin varied from 0.12 to 4 μM. The antibacterial effect was mainly bactericidal and fast (approximately 0.5 h after incubation) and was maintained even in stationary cells and mature MRSA biofilms. Melittin interacts synergistically with beta-lactams and aminoglycosides, and its ability to form pores in the membrane reverses the resistance of vancomycin-intermediate Staphylococcus aureus (VISA) to amoxicillin, and vancomycin. Its ability to induce resistance in vitro was absent, and melittin was stable in several conditions often associated with infected wounds. In vivo, aointment containing melittin reduced bacterial load and the content of pro-inflammatory cytokines, such as tumor necrosis factor-α, interleukin-6 (IL-6), and IL-1 beta. Collectively, these data point to melittin as a potential candidate for topical formulations aimed at the treatment of non-surgical infections caused by MRSA.pt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorshipOutra Agênciapt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE ALIMENTOSpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICASpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofEuropean Journal of Pharmaceutical Sciencespt_BR
dc.rightsAcesso Restritopt_BR
dc.subjectMethicillin-resistant Staphylococcus aureuspt_BR
dc.subjectMelittinpt_BR
dc.subjectWoundpt_BR
dc.subjectAntimicrobial peptidespt_BR
dc.subject.otherStaphylococcus aureuspt_BR
dc.subject.otherMelitinapt_BR
dc.subject.otherPotencial antibacterianopt_BR
dc.subject.otherPotencial farmacêuticopt_BR
dc.subject.otherPomadapt_BR
dc.titleIn-depth characterization of antibacterial activity of melittin against Staphylococcus aureus and use in a model of non-surgical MRSA-infected skin woundspt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.sciencedirect.com/science/article/pii/S0928098720303808pt_BR
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