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Please use this identifier to cite or link to this item: http://hdl.handle.net/1843/41867
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dc.creatorMateus Araújo Castro e Souzapt_BR
dc.creatorNaialy Fernandes Araújo Reispt_BR
dc.creatorIsabella Marques da Silva Leitept_BR
dc.creatorMaria Beatriz de Abreu Glóriapt_BR
dc.creatorGerson Antônio Pianettipt_BR
dc.creatorMaria Auxiliadora Parreiras Martinspt_BR
dc.creatorIsabella Cristina Plácido de Oliveira Pachecopt_BR
dc.creatorChristian Fernandespt_BR
dc.date.accessioned2022-05-20T21:50:41Z-
dc.date.available2022-05-20T21:50:41Z-
dc.date.issued2020-09-05-
dc.citation.volume188pt_BR
dc.citation.spage1pt_BR
dc.citation.epage8pt_BR
dc.identifier.doi10.1016/j.jpba.2020.113403pt_BR
dc.identifier.issn0731-7085pt_BR
dc.identifier.urihttp://hdl.handle.net/1843/41867-
dc.description.resumoAngiotensin II receptor antagonists are one of the most widely used classes of antihypertensive drugs. In this study, an HPLC fluorescence method after protein precipitation (PPT) extraction was developed and validated for determination of olmesartan, losartan, irbesartan, and valsartan in human plasma. The separation was carried out on a Luna cyano (250 × 4.6 mm i.d.; 5 μm particle size) column and the mobile phase was composed of acetonitrile and 0.1 % phosphoric acid in gradient elution, at a flow rate of 1.2 mL min−1. A PPT method was optimized by a two-level factorial design with triplicate at the central point. The parameters that could affect the extraction (sample volume and acetonitrile/plasma volume ratio) were evaluated and the method was compared to microextraction by packed sorbent (MEPS) and liquid-liquid extraction (LLE). The developed method allowed the simultaneous quantification of the analytes employing a simple and cheap sample preparation method and a short chromatographic run (13 min). This method was fully validated showing selectivity, precision, accuracy, and linearity over the range of 25.0–1500.0 ng mL−1 for olmesartan and valsartan, 25.0–2500.0 ng mL−1 for irbesartan, and 35.0–2500.0 ng mL−1 for losartan. Finally, the method was successfully applied in the analysis of human plasma from volunteers.pt_BR
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológicopt_BR
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Geraispt_BR
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorpt_BR
dc.description.sponsorshipOutra Agênciapt_BR
dc.languageengpt_BR
dc.publisherUniversidade Federal de Minas Geraispt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE ALIMENTOSpt_BR
dc.publisher.departmentFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOSpt_BR
dc.publisher.initialsUFMGpt_BR
dc.relation.ispartofJournal of Pharmaceutical and Biomedical Analysispt_BR
dc.rightsAcesso Restritopt_BR
dc.subjectAngiotensin receptor blockerspt_BR
dc.subjectLiquid chromatographypt_BR
dc.subjectFluorescencept_BR
dc.subjectProtein precipitationpt_BR
dc.subjectMicroextraction by packed sorbentpt_BR
dc.subjectLiquid-liquid extractionpt_BR
dc.subject.otherCromatografia líquidapt_BR
dc.subject.otherAnti-hipertensivospt_BR
dc.subject.otherMétodo de fluorescênciapt_BR
dc.titleA simple and sensitive HPLC-FL method for simultaneous determination of angiotensin II receptor antagonists in human plasmapt_BR
dc.typeArtigo de Periódicopt_BR
dc.url.externahttps://www.sciencedirect.com/science/article/pii/S0731708520312899pt_BR
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